|
| Status |
Public on Aug 08, 2006 |
| Title |
Per2Cry1 adrenal 54hrs DD B |
| Sample type |
RNA |
| |
|
| Source name |
Per2Cry1 mutant mouse whole adrenal 54hrs after lights off
|
| Organism |
Mus musculus |
| Characteristics |
strain: Per2Brdm1/Cry1-/- (C57BL6x129Sv) gender: male age: 3 months tissue: whole adrenal gland
|
| Treatment protocol |
animals were LD entrained for two weeks and subsequently released into DD; whole adrenals were dissected at the specified time points after
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Micro Kit (QIAGEN)
|
| Label |
biotin
|
| Label protocol |
http://www.rzpd.de/protocols
|
| |
|
| Hybridization protocol |
http://www.rzpd.de/protocols
|
| Scan protocol |
http://www.rzpd.de/protocols
|
| Description |
Per2/Cry1 animals are circadian clock-deficient. This set was used to identify clock regulated genes in the adrenal that are not necessarily rhythmic.
|
| Data processing |
expression values for the whole series were normalized (PM/MM model) using DChip 1.3 software
|
| |
|
| Submission date |
Feb 14, 2006 |
| Last update date |
Jul 27, 2006 |
| Contact name |
Henrik Oster |
| E-mail(s) |
henrik.oster@uni-luebeck.de
|
| Organization name |
University of Lübeck
|
| Department |
CBBM
|
| Street address |
Marie Curie Street
|
| City |
Lübeck |
| ZIP/Postal code |
23562 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE4239 |
Transcriptome regulation in the adrenal gland of circadian clock deficient Per2Cry1 double mutant mice |
| GSE4253 |
Wildtype and Per2Cry1 double mutant mice: temporal gene expression profiling of adrenal gland |
|
