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Sample GSM966950 Query DataSets for GSM966950
Status Public on Jul 17, 2012
Title Synaptosomal RNA from High Dose treated (EUK8) Constitutive Sod2 knock out HKO-4
Sample type RNA
 
Source name synaptosomes from frontal cortex
Organism Mus musculus
Characteristics age: 17-21 days
genotype: Sod2 KO
treatment: High (30mg/kg) dose EUK189
sample type: synaptosomes
genetic background: CD1
Extracted molecule total RNA
Extraction protocol Trizol
Label Cy3
Label protocol Samples which passed quality were amplified one round and biotin-labeled, using the Illumina TotalPrep Kit (Ambion) according to the manufacturer's instructions. Concentration and quality were assessed using the Nanodrop Spectrophotometer and the Agilent Bioanalyzer Nano Chip System.
 
Hybridization protocol Labeled cRNA samples were hybridized to Human Ref-8 BeadChips (Illumina) according to the manufacturer's instructions, using equipment specified by the manufacturer (Illumina).
Scan protocol Processed arrays were read using a BeadStation array reader (Illumina) according to the manufacturer's instructions.
Description HKO-4
Synaptosomal RNA from High Dose treated (EUK8) Constitutive Sod2 knock out
4432281030_F
Data processing quantile normalization
 
Submission date Jul 16, 2012
Last update date Jul 30, 2012
Contact name Serban Ciotlos
E-mail(s) smelov@buckinstitute.org
Phone 4085068553
Organization name Buck Institute for Research on Aging
Lab Melov Lab
Street address 8001 Redwood Blvd
City Novato
State/province CA
ZIP/Postal code 94945
Country USA
 
Platform ID GPL6885
Series (1)
GSE39372 Proteogenomics of synaptosomal mitochondrial oxidative stress

Data table header descriptions
ID_REF
VALUE quantile normalized log2 signal

Data table
ID_REF VALUE
ILMN_1248788 6.002367351
ILMN_2707227 5.8014008
ILMN_2896528 10.14255981
ILMN_2721178 8.598991157
ILMN_1227723 6.473301117
ILMN_3033922 8.358890049
ILMN_3092673 9.86974135
ILMN_2730714 8.811123971
ILMN_3162224 8.267636158
ILMN_2816356 5.805584284
ILMN_2808939 7.38835957
ILMN_2634564 7.150150959
ILMN_1216623 7.494047202
ILMN_1215157 8.151974004
ILMN_1228777 5.923071193
ILMN_2737647 6.058853902
ILMN_1216425 6.020596601
ILMN_2734484 6.979531475
ILMN_2652961 5.664080286
ILMN_2952292 6.491100826

Total number of rows: 25697

Table truncated, full table size 624 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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