|
| Status |
Public on Jul 17, 2012 |
| Title |
Synaptosomal RNA from High Dose treated (EUK8) Constitutive Sod2 knock out HKO-6 |
| Sample type |
RNA |
| |
|
| Source name |
synaptosomes from frontal cortex
|
| Organism |
Mus musculus |
| Characteristics |
age: 17-21 days
genotype: Sod2 KO
treatment: High (30mg/kg) dose EUK189
sample type: synaptosomes
genetic background: CD1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Cy3
|
| Label protocol |
Samples which passed quality were amplified one round and biotin-labeled, using the Illumina TotalPrep Kit (Ambion) according to the manufacturer's instructions. Concentration and quality were assessed using the Nanodrop Spectrophotometer and the Agilent Bioanalyzer Nano Chip System.
|
| |
|
| Hybridization protocol |
Labeled cRNA samples were hybridized to Human Ref-8 BeadChips (Illumina) according to the manufacturer's instructions, using equipment specified by the manufacturer (Illumina).
|
| Scan protocol |
Processed arrays were read using a BeadStation array reader (Illumina) according to the manufacturer's instructions.
|
| Description |
HKO-6
Synaptosomal RNA from High Dose treated (EUK8) Constitutive Sod2 knock out
4432281037_G
|
| Data processing |
quantile normalization
|
| |
|
| Submission date |
Jul 16, 2012 |
| Last update date |
Jul 30, 2012 |
| Contact name |
Simon Melov |
| Organization name |
Buck Institute for Research on Aging
|
| Lab |
Melov Lab
|
| Street address |
8001 Redwood Blvd
|
| City |
Novato |
| State/province |
CA |
| ZIP/Postal code |
94945 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE39372 |
Proteogenomics of synaptosomal mitochondrial oxidative stress |
|
