|
| Status |
Public on Dec 31, 2012 |
| Title |
DnMaml;MyoD mutant rep2 |
| Sample type |
RNA |
| |
|
| Source name |
embryonic back muscle progenitor cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: mixed C57BL/6, 129/Sv and 129/Ola.
genotype: Pax3cre/+;DnMamlflox/flox;MyoD-/-
tissue: skeletal muscle
cell type: progenitor cell
developmental stage: E17.5
|
| Growth protocol |
Muscle progenitor cells were purified by FACS sorting from primary samples for transcription analysis.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up with the RNeasy MinElute Cleanup Kit (Qiagen) in accordance with the prescribed protocol provided with the kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina TotalPrep RNA Amplification Kit (Ambion) and the RNeasy MinElute Cleanup Kit (Qiagen)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
5535688043_E
|
| Data processing |
The data were normalised using quantile normalisation with Illumina Genome Studio software (version 2010.1) with the Gene Expression Module (version 1.6.0).
|
| |
|
| Submission date |
Jul 16, 2012 |
| Last update date |
Dec 31, 2012 |
| Contact name |
Dominique Bröhl |
| Organization name |
Max-Delbrück-Center for Molecular Medicine
|
| Department |
Research Program Function and Dysfunction of the Nervous System
|
| Lab |
Developmental Biology/Signal Transduction Group
|
| Street address |
Robert-Rössle-Str. 10
|
| City |
Berlin |
| State/province |
Berlin |
| ZIP/Postal code |
13092 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE39379 |
Notch signaling/MyoD double deficient embryonic muscle progenitors |
|
