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Sample GSM967070 Query DataSets for GSM967070
Status Public on Dec 31, 2012
Title DnMaml;MyoD mutant rep4
Sample type RNA
 
Source name embryonic back muscle progenitor cells
Organism Mus musculus
Characteristics strain: mixed C57BL/6, 129/Sv and 129/Ola.
genotype: Pax3cre/+;DnMamlflox/flox;MyoD-/-
tissue: skeletal muscle
cell type: progenitor cell
developmental stage: E17.5
Growth protocol Muscle progenitor cells were purified by FACS sorting from primary samples for transcription analysis.
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up with the RNeasy MinElute Cleanup Kit (Qiagen) in accordance with the prescribed protocol provided with the kit.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Illumina TotalPrep RNA Amplification Kit (Ambion) and the RNeasy MinElute Cleanup Kit (Qiagen)
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description 5535688057_E
Data processing The data were normalised using quantile normalisation with Illumina Genome Studio software (version 2010.1) with the Gene Expression Module (version 1.6.0).
 
Submission date Jul 16, 2012
Last update date Dec 31, 2012
Contact name Dominique Bröhl
Organization name Max-Delbrück-Center for Molecular Medicine
Department Research Program Function and Dysfunction of the Nervous System
Lab Developmental Biology/Signal Transduction Group
Street address Robert-Rössle-Str. 10
City Berlin
State/province Berlin
ZIP/Postal code 13092
Country Germany
 
Platform ID GPL6885
Series (1)
GSE39379 Notch signaling/MyoD double deficient embryonic muscle progenitors

Data table header descriptions
ID_REF
VALUE quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1248788 7.09569 0.2656642
ILMN_2707227 28.88157 0.0914787
ILMN_2896528 8120.928 0
ILMN_2721178 1429.263 0
ILMN_1227723 235.8495 0
ILMN_3033922 2047.47 0
ILMN_3092673 8303.771 0
ILMN_2730714 9340.21 0
ILMN_3162224 4124 0
ILMN_2816356 22.28874 0.1203008
ILMN_2808939 914.3517 0
ILMN_2634564 729.665 0
ILMN_1216623 932.9105 0
ILMN_1215157 1610.286 0
ILMN_1228777 -4.744576 0.4824561
ILMN_2737647 10.99785 0.2155388
ILMN_1216425 4.542935 0.302005
ILMN_2734484 249.6934 0
ILMN_2652961 8.841962 0.235589
ILMN_2952292 254.9291 0

Total number of rows: 25697

Table truncated, full table size 731 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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