|
| Status |
Public on Jan 17, 2013 |
| Title |
FAP patient E |
| Sample type |
RNA |
| |
|
| Source name |
patient E
|
| Organism |
Homo sapiens |
| Characteristics |
individual: patient E
marker: FAP
disease state: human CRC
cell population: fibroblasts
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was processed and amplified as previously described(Gonzalez-Roca et al., 2010).
|
| Label |
biotin
|
| Label protocol |
RNA was labeled (biotin) using GeneChip Mapping 10K Xba Assay Kit (Affymetrix; catalog # 900441), according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Affymetrix HG-U133 +PM arrays were hybridized with 8 ug of labeled, amplified dsDNA, washed, stained and scanned according to the protocol described in Affymetrix GeneChip® Expression Analysis Manual.
|
| Scan protocol |
Scanning was done on an Affymetrix GeneChip Scanner 7G
|
| Description |
expression data from fibroblasts
|
| Data processing |
We used RMA background correction, quantile normalization and RMA summarization(Gautier et al., 2004).
|
| |
|
| Submission date |
Jul 16, 2012 |
| Last update date |
Jan 17, 2013 |
| Contact name |
Alexandre CALON |
| E-mail(s) |
alexandre.calon@gmail.com
|
| Organization name |
Hospital del Mar Medical Research Institute (IMIM)
|
| Street address |
Dr. Aiguader, 88
|
| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
|
| Platform ID |
GPL13158 |
| Series (2) |
| GSE39396 |
Expression profiles of cell populations purified from human CRC (4 ways) |
| GSE39397 |
Human CRC cell populations |
|
