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Sample GSM96793

Query DataSets for GSM96793

Status

Public on Jun 15, 2006

Title

Treg control

Sample type

RNA

Source name

CD4+CD25high

Organism

Homo sapiens

Characteristics

CD4+CD25high T-Lymphocytes derived from human PBL (control group)

Extracted molecule

total RNA

Label

Biotin-SA-PE

Description

Peripheral blood was obtained from healthy adult donors. Human PBL were purified from peripheral blood by bicoll gradient centrifugation followed by plastic adherence to deplete monocytes. Adult peripheral CD4+CD25+ cells were first enriched using MACS beads (Miltenyi Biotech) and subsequently CD4+CD25high cells were sorted with a FACS-Diva™ cell sorter (BD Biosciences).

Data processing

MAS 5.0

Submission date

Feb 15, 2006

Last update date

Feb 15, 2006

Contact name

Robert Geffers

E-mail(s)

robert.geffers@helmholtz-hzi.de

Phone

+49 531-6181-3058

Organization name

HCI - Helmholtz Centre for Infection Research

Department

Dep. Molecular Bacteriology

Lab

Genome Analytics

Street address

Inhoffenstr. 7

City

Braunschweig

ZIP/Postal code

38124

Country

Germany

Platform ID

GPL571

Series (1)

GSE4243

Differential expression pattern in AICD resistent TReg vs. CD4+CD25high TReg cells

Data table header descriptions
ID_REF
VALUE	Signal Intensity
ABS_CALL	MAS5 signal significance estimation expressed as P (Present), A (Absent), M (Marginal)
DETECTION P-VALUE	Mas5 signal significance p-Value

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
AFFX-BioB-5_at	116.3	P	0.002275
AFFX-BioB-M_at	186.5	P	0.002275
AFFX-BioB-3_at	120.8	P	0.012547
AFFX-BioC-5_at	419.7	P	0.000044
AFFX-BioC-3_at	498.5	P	0.00007
AFFX-BioDn-5_at	1059.6	P	0.000095
AFFX-BioDn-3_at	2189.4	P	0.000044
AFFX-CreX-5_at	2411	P	0.000044
AFFX-CreX-3_at	4531.6	P	0.000044
AFFX-DapX-5_at	18.9	A	0.645547
AFFX-DapX-M_at	3.8	A	0.860518
AFFX-DapX-3_at	15.1	A	0.60308
AFFX-LysX-5_at	29.3	A	0.327079
AFFX-LysX-M_at	10.6	A	0.760937
AFFX-LysX-3_at	28.7	A	0.216524
AFFX-PheX-5_at	24.8	A	0.327079
AFFX-PheX-M_at	4.8	A	0.659339
AFFX-PheX-3_at	54.5	A	0.300606
AFFX-ThrX-5_at	10.1	A	0.749204
AFFX-ThrX-M_at	9	A	0.814869

Total number of rows: 22277

Table truncated, full table size 581 Kbytes.

Supplementary data files not provided