|
| Status |
Public on Jan 20, 2015 |
| Title |
Brain-Alzheimer's disease PSEN1 mutation carrier patient-2 |
| Sample type |
RNA |
| |
|
| Source name |
Frozen brain tissue: posterior cingulate at thalamus level
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
age at death: 53
post-mortem interval (hours): 5:15
apoe genotype: 3/3
psen1 mutation: M139T
neuropathological data (braak stage): A3, B3, C3 + LBD limbic predominant
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from frozen brain tissue (posterior cingulate area) using the RNeasy Lipid Tissue Mini Kit (Qiagen)
|
| Label |
Biotin
|
| Label protocol |
Labeled ssDNA was prepared according to the standard Affymetrix Terminal labeling Kit protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 ug of ssDNA were hybridized for 16 hr at 45C following Affymetrix standard procedures
|
| Scan protocol |
GeneChips were scanned according to the standard Affymetrix protocol (Affymetrix Genechip Command Console program 3.1.0.1228, scanner PlateScanner M10)
|
| Description |
Gene expression data of final stages of Alzheimer's disease
|
| Data processing |
Raw data was background adjusted, normalized and summarized by Robust Multi-array Analysis (RMA) using the Affy package (version 1.30.0) from Bioconductor on R language (version 2.13.2).
|
| |
|
| Submission date |
Jul 17, 2012 |
| Last update date |
Jan 20, 2015 |
| Contact name |
Anna Antonell |
| E-mail(s) |
antonell@clinic.ub.es
|
| Organization name |
Hospital Clínic Barcelona
|
| Department |
Unitat d'Alzheimer i altres transtorns cognitius
|
| Lab |
Malalties neurodegeneratives
|
| Street address |
C/Casanova, 143
|
| City |
Barcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL11532 |
| Series (1) |
| GSE39420 |
Gene expression profile of sporadic and PSEN1 early-onset Alzheimer’s Disease |
|
