|
Status |
Public on Jun 15, 2006 |
Title |
rTreg |
Sample type |
RNA |
|
|
Source name |
CD95L mediated cell death resistant Treg
|
Organism |
Homo sapiens |
Characteristics |
CD4+CD25+CD45RO(low) CD31(high) sorted T-Lymphocytes from PBL are resistent to CD95L-mediated cell death (AICD resisent group)
|
Extracted molecule |
total RNA |
Label |
Biotin-SA-PE
|
|
|
Description |
Peripheral blood was obtained from 8 healthy adult donors. Human PBL were purified from peripheral blood by bicoll gradient centrifugation followed by plastic adherence to deplete monocytes. Adult peripheral CD4+CD25+ cells were first enriched using MACS beads (Miltenyi Biotech) and subsequently CD4+CD25high CD45RO(low)CD31(high)cells were sorted with a FACS-Diva™ cell sorter (BD Biosciences).
|
Data processing |
MAS 5.0
|
|
|
Submission date |
Feb 15, 2006 |
Last update date |
Feb 15, 2006 |
Contact name |
Robert Geffers |
E-mail(s) |
robert.geffers@helmholtz-hzi.de
|
Phone |
+49 531-6181-3058
|
Organization name |
HCI - Helmholtz Centre for Infection Research
|
Department |
Dep. Molecular Bacteriology
|
Lab |
Genome Analytics
|
Street address |
Inhoffenstr. 7
|
City |
Braunschweig |
ZIP/Postal code |
38124 |
Country |
Germany |
|
|
Platform ID |
GPL571 |
Series (1) |
GSE4243 |
Differential expression pattern in AICD resistent TReg vs. CD4+CD25high TReg cells |
|