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Status
Public on Feb 17, 2006
Title
Cumulus Oocyte Complex eCG-treated 48h, hCG 16h replicate 2
Sample type
RNA
Source name
cumulus-oocyte complex
Organism
Mus musculus Characteristics
23 d.o. C57BL/6 female, eCG stimulated 48h - hCG stimulated 16h, cumulus-oocyte complex
Extracted molecule
total RNA
Extraction protocol
Immature female mice were stimulated with eCG for 48h then stimulated for 16h with hCG. Cumulus-oocyte complexes were isolated from the oviduct by needle puncture, pooled, then lysed using the RNeasy kit (Qiagen) according to the manufacturer's instructions .Total RNA was extracted from the tissue lysate using an RNeasy kit (Qiagen).
Label
biotin
Label protocol
Approximately 8 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Wild type eCG-, hCG-primed (16h) cumulus-oocyte complex
Data processing
Affymetrix GeneChip Mouse Genome 430 2.0 Array
Submission date
Feb 17, 2006
Last update date
Aug 28, 2018
Contact name
JoAnne S Richards
E-mail(s)
joanner@bcm.tmc.edu Phone
713-798-6238
Organization name
Baylor College of Medicine
Department
Molecular and Cellular Biology
Street address
One Baylor Plaza
City
Houston
State/province
TX
ZIP/Postal code
77030
Country
USA
Platform ID
GPL1261 Series (1)
GSE4260 Cumulus-oocyte complex temporal expression
Relations Reanalyzed by
GSE119085
Data table header descriptions ID_REF VALUE RMA
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE 1415670_at
11.20342077
P
0.001641799
1415671_at
11.28745504
P
0.002180626
1415672_at
11.84299634
P
0.001641799
1415673_at
8.575158402
P
0.002180626
1415674_a_at
10.76078752
P
0.001641799
1415675_at
9.746179893
P
0.001641799
1415676_a_at
13.16541324
P
0.001641799
1415677_at
9.828466198
P
0.001641799
1415678_at
12.13245527
P
0.001641799
1415679_at
11.54116661
P
0.001641799
1415680_at
11.44601538
P
0.001641799
1415681_at
10.55124824
P
0.001641799
1415682_at
8.778570708
P
0.001641799
1415683_at
12.30819706
P
0.001641799
1415684_at
8.983796123
P
0.001641799
1415685_at
9.599091482
P
0.001641799
1415686_at
10.86841971
P
0.001641799
1415687_a_at
13.75458948
P
0.001641799
1415688_at
11.45347844
P
0.001641799
1415689_s_at
11.2952517
P
0.001641799
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