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Sample GSM973451 Query DataSets for GSM973451
Status Public on Jul 26, 2012
Title +/+ hippocampus 3
Sample type RNA
 
Source name hippocampus, transgenic
Organism Mus musculus
Characteristics strain: C57BL/6
genotype: transgenic
tissue: hippocampus
Extracted molecule total RNA
Extraction protocol RNA was extracted by using RNeasy Midi Kit (Qiagen) following manufacturers instructions for animal tissues.
Label biotin
Label protocol Biotinylated cDNA was prepared according to the Affymetrix Whole Transkript Sense Labelling protocol from100 ng total RNA according to the manufacturer
 
Hybridization protocol Following fragmentation, 15 ug of cDNA were hybridized for 16 hr at 45°C on GeneChip MoGene-1.0-st-v1 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GCS3000 gene chip scanner with GCOS 1.4.
Data processing The data were analysed using Bioconductor package 'xps' under R-2.10.0. Background correction, normalization and probe summarization was performed with RMA for each tissue separately using the core transcript annotation.
 
Submission date Jul 25, 2012
Last update date Jul 26, 2012
Contact name Stephan Direnberger
E-mail(s) direnberger@bio.lmu.de
Organization name Ludwig-Maximilians University
Street address Grosshaderner Str. 2
City Martinsried
ZIP/Postal code 82152
Country Germany
 
Platform ID GPL11533
Series (1)
GSE39632 Gene Expression profiling of transgenic mice expressing the genetically encoded calcium indicator TN-XXL in muscle and brain tissues

Data table header descriptions
ID_REF
VALUE RMA log2 transformed signal values.

Data table
ID_REF VALUE
10338001 9.76129691
10338003 8.233988691
10338004 7.788529114
10338017 11.60239731
10338025 6.570831755
10338026 11.65919764
10338029 7.526499239
10338035 6.600561166
10338036 7.804247207
10338037 2.041579432
10338041 9.457711245
10338042 8.485213466
10338044 10.31292832
10338047 3.510772078
10338056 1.940392301
10338059 11.26418453
10338060 2.007335458
10338064 2.186716058
10338065 3.291985103
10338066 1.983013647

Total number of rows: 35382

Table truncated, full table size 721 Kbytes.




Supplementary file Size Download File type/resource
GSM973451_E10R025f03.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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