|
Status |
Public on Jul 25, 2012 |
Title |
liver_wild type_replicate3 |
Sample type |
RNA |
|
|
Source name |
liver_wild type
|
Organism |
Mus musculus |
Characteristics |
tissue: liver genotype/variation: wild type
|
Growth protocol |
Mice were housed in Type II L cages (365x207x140 mm, floor area 530 cm2; IVC-based)at an environmental temperature 20-24°C, humidity: 55±10%and under 12/12 h light/dark cycle with food and water ad libitum.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted by using RNeasy Midi Kit (Qiagen) following manufacturers instructions for animal tissues.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse [HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GCS3000 gene chip scanner with GCOS 1.4.
|
Description |
E10R028a03
|
Data processing |
The data were analysed using Bioconductor package 'affy' under R-2.7.0. Background correction, normalization and probe summarization was performed with RMA (function just.rma()).
|
|
|
Submission date |
Jul 25, 2012 |
Last update date |
Jul 25, 2012 |
Contact name |
Michael H. Walter |
E-mail(s) |
michael.walter@agilent.com
|
Organization name |
University of Tuebingen
|
Department |
Department of Medical Genetics
|
Lab |
The Microarray Facility Tübingen
|
Street address |
Calwer Str. 7
|
City |
Tuebingen |
ZIP/Postal code |
72076 |
Country |
Germany |
|
|
Platform ID |
GPL11180 |
Series (1) |
GSE39640 |
ATXN2-CAG42 sequesters PABPC1 into insolubility and induces FBXW8 in cerebellum of old ataxic knock-in mice |
|