|
| Status |
Public on Jul 25, 2012 |
| Title |
brainstem_Atxn2 Knock-In_replicate2 |
| Sample type |
RNA |
| |
|
| Source name |
brainstem_Atxn2 Knock-In
|
| Organism |
Mus musculus |
| Characteristics |
tissue: brainstem
genotype/variation: Atxn2 Knock-In
|
| Growth protocol |
Mice were housed in Type II L cages (365x207x140 mm, floor area 530 cm2; IVC-based)at an environmental temperature 20-24°C, humidity: 55±10%and under 12/12 h light/dark cycle with food and water ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted by using RNeasy Midi Kit (Qiagen) following manufacturers instructions for animal tissues.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse [HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GCS3000 gene chip scanner with GCOS 1.4.
|
| Description |
E10R028f02
|
| Data processing |
The data were analysed using Bioconductor package 'affy' under R-2.7.0. Background correction, normalization and probe summarization was performed with RMA (function just.rma()).
|
| |
|
| Submission date |
Jul 25, 2012 |
| Last update date |
Jul 25, 2012 |
| Contact name |
Michael H. Walter |
| E-mail(s) |
michael.walter@agilent.com
|
| Organization name |
University of Tuebingen
|
| Department |
Department of Medical Genetics
|
| Lab |
The Microarray Facility Tübingen
|
| Street address |
Calwer Str. 7
|
| City |
Tuebingen |
| ZIP/Postal code |
72076 |
| Country |
Germany |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE39640 |
ATXN2-CAG42 sequesters PABPC1 into insolubility and induces FBXW8 in cerebellum of old ataxic knock-in mice |
|
