|
| Status |
Public on Oct 01, 2012 |
| Title |
Ox-ARP, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Neuro 2a cells, H2O2, ARP-derivatized RNA
|
| Organism |
Mus musculus |
| Characteristics |
cell line: Neuro 2a
strain: C57BL/6J
treatment: hydrogen peroxide
pull-down process: yes
|
| Treatment protocol |
Neuro 2a cells received hydrogen peroxide treatment or no treatment as a control.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA isolated by TRIzol was derivatized with ARP on abasic sites. After the RNA was incubated with streptavidin magnetic beads and then washed with stringent buffer conditions, oxidized RNA was specifically isolated by pull-down.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion TotalPrep kit for Illumina arrays. Cy3-Streptavidin was incorporated during the Wash and Block protocol as a fluor for scanning.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
5541914022_H
Replicate 2 of 4.
|
| Data processing |
The data were normalized using average normalization in GenomeStudio v2010.2.
|
| |
|
| Submission date |
Jul 25, 2012 |
| Last update date |
Oct 01, 2012 |
| Contact name |
Penn State COM |
| Organization name |
Penn State College of Medicine
|
| Department |
Genome Sciences Facility
|
| Street address |
500 University Drive
|
| City |
Hershey |
| State/province |
PA |
| ZIP/Postal code |
17033 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE39641 |
Genome-Wide Study Based On Abasic Site Revealed Selective RNA Oxidation In Neuro 2a Cells |
|
