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Status |
Public on Dec 31, 2012 |
Title |
BMDM-2 |
Sample type |
RNA |
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|
Source name |
bone marrow derived macrophages
|
Organism |
Mus musculus |
Characteristics |
treatment: control media strain: B6.Cg-Tg(CAG-DsRed*MST)1Nagy/J (Jackson stock #006051)
|
Treatment protocol |
BMDM were induced by LPS or IL4 for 24 hours.
|
Growth protocol |
Bone marrow cells were plated on dish for 7 days in the presence of M-CSF or plated on endothelial monolayer for 7 days with HSC media.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using RNeasy Mini Kit (Invitrogen). Integrity of the RNA was evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Also, CA, USA) and purity/concentration was determined using a NanoDrop 8000 Spectrophotometer (Thermo Scientific, Wilminton, DE, USA).
|
Label |
Cy3
|
Label protocol |
RNA was amplified into cRNA and labeled with Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technologies).
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|
|
Hybridization protocol |
Samples were hybridized to Agilent mouse 8X60k array (Agilent Technologies).
|
Scan protocol |
The acquisition of array image was undertaken by using Agilent Scan Control and Agilent Feature Extraction 10.7 software.
|
Data processing |
Raw data were analyzed using Partek Genomics Suite 6.4 and normalized by using RMA algorithm. Thresholds for selecting significant genes were set at >= 2-fold and FDR corrected p<0.05. Genes which met both criteria were considered as having significant changes.
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|
|
Submission date |
Jul 26, 2012 |
Last update date |
Dec 31, 2012 |
Contact name |
Calvin Pan |
Organization name |
UCLA
|
Street address |
675 Charles Young Dr South
|
City |
Los Angeles |
ZIP/Postal code |
90095 |
Country |
USA |
|
|
Platform ID |
GPL10787 |
Series (1) |
GSE39660 |
Endothelial Cells Provide an Instructive Niche for the Differentiation and Functional Polarization of M2-like Macrophages |
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