|
| Status |
Public on Dec 31, 2012 |
| Title |
BMDM_IL4-2 |
| Sample type |
RNA |
| |
|
| Source name |
bone marrow derived macrophages
|
| Organism |
Mus musculus |
| Characteristics |
treatment: IL4
strain: B6.Cg-Tg(CAG-DsRed*MST)1Nagy/J (Jackson stock #006051)
|
| Treatment protocol |
BMDM were induced by LPS or IL4 for 24 hours.
|
| Growth protocol |
Bone marrow cells were plated on dish for 7 days in the presence of M-CSF or plated on endothelial monolayer for 7 days with HSC media.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNeasy Mini Kit (Invitrogen). Integrity of the RNA was evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Also, CA, USA) and purity/concentration was determined using a NanoDrop 8000 Spectrophotometer (Thermo Scientific, Wilminton, DE, USA).
|
| Label |
Cy3
|
| Label protocol |
RNA was amplified into cRNA and labeled with Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technologies).
|
| |
|
| Hybridization protocol |
Samples were hybridized to Agilent mouse 8X60k array (Agilent Technologies).
|
| Scan protocol |
The acquisition of array image was undertaken by using Agilent Scan Control and Agilent Feature Extraction 10.7 software.
|
| Data processing |
Raw data were analyzed using Partek Genomics Suite 6.4 and normalized by using RMA algorithm. Thresholds for selecting significant genes were set at >= 2-fold and FDR corrected p<0.05. Genes which met both criteria were considered as having significant changes.
|
| |
|
| Submission date |
Jul 26, 2012 |
| Last update date |
Dec 31, 2012 |
| Contact name |
Calvin Pan |
| Organization name |
UCLA
|
| Street address |
675 Charles Young Dr South
|
| City |
Los Angeles |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL10787 |
| Series (1) |
| GSE39660 |
Endothelial Cells Provide an Instructive Niche for the Differentiation and Functional Polarization of M2-like Macrophages |
|
