|
| Status |
Public on Jul 28, 2012 |
| Title |
zebrafish embryo at 36 hpf EET, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
pooled mRNA from whole embryo at 36 hpf treated with 5uM 11,12-EET
|
| Organism |
Danio rerio |
| Characteristics |
tissue: Dechorionated embryo
genotype: Wild type AB
age: embryos at 36 hours post fertilization
treatment: 5uM 11,12-EET
|
| Treatment protocol |
Embryos were treated with DMSO or 5 uM 11,12-EET between 24-36 hpf. Embryos were dechorionated with pronase and homogenized in the Trizol solution (GibcoBRL). 25 embryos were used per treatment group.
|
| Growth protocol |
embryos were synchronized for the fertilization time and grew at 28 degree the whole time.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Zebrafish Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
The data were analyzed with Goldenspike (http://www2.ccr.buffalo.edu/halfon/spike/). This R package background-corrects, normalizes, multiple-tests, and computes the sample statistics below.
|
| |
|
| Submission date |
Jul 27, 2012 |
| Last update date |
Jul 28, 2012 |
| Contact name |
Anthony Dominc DiBiase |
| E-mail(s) |
adibiase@enders.tch.harvard.edu
|
| Organization name |
Children's Hospital Boston
|
| Department |
Hem/Onc
|
| Lab |
Zon Lab
|
| Street address |
KFRB, 1 Blackfan St, Suite 8216
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE39707 |
Zebrafish whole embryos at 36 hpf treated with DMSO or 5uM 11,12-EET |
|
