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Sample GSM978508 Query DataSets for GSM978508
Status Public on Apr 01, 2013
Title 8_221_T_H
Sample type RNA
 
Source name Tak1/Caspase8 LPC double knockout mice
Organism Mus musculus
Characteristics genotype: Tak1/Caspase8 LPC double knockout mice
gender: male
tissue: liver
age: 6 weeks
Growth protocol Liver tissue, excised of 6 week old male mice
Extracted molecule total RNA
Extraction protocol RNA was extracted from snap frozen tissue using the Qiagen RNeasy Kit
Label biotin
Label protocol Samples were amplified and labelled using the Ambion® WT Expression Kit, combined with the GeneChip® WT Terminal Labeling and Controls Kit according to the manufacturers instructions.
 
Hybridization protocol Samples were hybridized using Affymetrix hybridization kit materials • Heat cocktails at 99° for 5 minutes, then 45° for 5 minutes centrifuge at max speed for 1 minute • Transfer 80μl of hyb solution to each array, then tape holes • Hybridize 16 hours at 45° at 60rpm • Fluidics washing is FS450_00007
Scan protocol according to Affymetrix protocol - Affymetrix Gene ChIP Scanner 3000 7G
Data processing normalization method: RMA; raw expression threshold: 150, dabg p: 0.05; expression data format: log2
 
Submission date Jul 30, 2012
Last update date Jun 06, 2013
Contact name Bernd Denecke
E-mail(s) bernd.denecke@rwth-aachen.de
Phone +49 241 8089918
Organization name RWTH Aachen University
Department IZKF Aachen
Street address Pauwelsstrasse 30
City Aachen
State/province NRW
ZIP/Postal code 52074
Country Germany
 
Platform ID GPL10740
Series (1)
GSE39750 Programmed necrosis and apoptosis differentially regulate cancer development and biliary homeostasis in the liver

Data table header descriptions
ID_REF
VALUE RMA signals estimated from Alt-Analyze

Data table
ID_REF VALUE
10344615 6.88997
10344617 2.79545
10344625 9.32108
10344626 12.17028
10344627 10.67969
10344628 12.29578
10344629 10.50648
10344630 12.17405
10344631 12.49792
10344632 12.72911
10344634 9.68484
10344636 9.91620
10344638 8.62616
10344639 11.04825
10344640 11.75202
10344641 9.88422
10344642 9.31944
10344643 7.96015
10344644 9.14429
10344645 9.83725

Total number of rows: 223771

Table truncated, full table size 3756 Kbytes.




Supplementary file Size Download File type/resource
GSM978508_8_221_T_H_MoGene-1_0-st-v1_.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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