|
| Status |
Public on Aug 08, 2012 |
| Title |
B cell of TG mouse 1 |
| Sample type |
RNA |
| |
|
| Source name |
B cells of transgenic mice
|
| Organism |
Mus musculus |
| Characteristics |
genetic background: Balb/C
age: 6-8 wk
gender: male
cell type: B cell from lymph nodes
genotype: miR-146a transgenic
|
| Extracted molecule |
total RNA |
| Extraction protocol |
B cells were isolated from lymph nodes with untouched magnetic separated assay, and dead cells were depleted with Dead Cell Removal Kit (Miltenyi Biotec). Cells were lysated with TRIzol reagent (Invitrogen). Transfer upper phase containing the RNA (final ethanol concentration of 35%) to a RNeasy spin cartridge and follow the manufacture’s guide (Qiangen).
|
| Label |
biotin
|
| Label protocol |
reference to the manual of Affymetrix (GeneAtlas™ 3’ IVT Express Kit)
|
| |
|
| Hybridization protocol |
reference to the manual of Affymetrix (GeneAtlas™ 3’ IVT Express Kit)
|
| Scan protocol |
reference to the manual of Affymetrix (GeneAtlas™ 3’ IVT Express Kit), follow the manuscipts of GeneAtlas Workstation.
|
| Data processing |
Command Console CHP file version 1, affymetrix-algorithm-name = rma, affymetrix-algorithm-version = 1.0, affymetrix-array-type = HT_MG-430_PM
|
| |
|
| Submission date |
Jul 31, 2012 |
| Last update date |
Aug 08, 2012 |
| Contact name |
qiuye guo |
| E-mail(s) |
tonyguo77@gmail.com
|
| Organization name |
The military medical university
|
| Street address |
GAO TAN YAN
|
| City |
CHONGQING |
| ZIP/Postal code |
510515 |
| Country |
China |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE39779 |
Profiling study of B cells from lymph nodes in miR-146a over-expression transgenic mice |
|
