|
Status |
Public on Aug 02, 2012 |
Title |
Abcg8 knockout testes, 4573 |
Sample type |
RNA |
|
|
Source name |
testes, Abcg8 knockout
|
Organism |
Mus musculus |
Characteristics |
strain/background: C57BL/6J genotype: Abcg8 knockout gender: male developmental stage: adult age: 10-12 weeks tissue: testes
|
Growth protocol |
Mice were fed rodent chow. All mice were between 10-12 weeks of age.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted and purified using RiboPure RNA Isolation (Ambion). Total RNA samples were quantitated by UV spectrophotometry. Quality of total RNA was assessed using an Agilent Bioanalyzer.
|
Label |
Digoxigenin
|
Label protocol |
First and second strand cDNA was prepared from the total RNA samples. Digoxigenin-labeled cRNA target was prepared from the DNA template and verified on the Bioanalyzer.
|
|
|
Hybridization protocol |
cRNA was fragmented to uniform size and verified on the Bioanalyzer. Applied Biosystems Mouse Genome Survey microarrays were hybridized with the cRNA target and treated for chemiluminescence detection.
|
Scan protocol |
Arrays were imaged and processed on the AB1700 Microarray Analyzer.
|
Description |
4573
|
Data processing |
Data was analyzed with GeneSpring GX software. Intensity values were normalized to the median intensity of each array.
|
|
|
Submission date |
Aug 01, 2012 |
Last update date |
Aug 02, 2012 |
Contact name |
Michael Falduto |
E-mail(s) |
mfalduto@genusbiosystems.com
|
Phone |
847-291-9602
|
Organization name |
GenUs BioSystems, Inc.
|
Street address |
1808 Janke, Unit M
|
City |
Northbrook |
State/province |
IL |
ZIP/Postal code |
60062 |
Country |
USA |
|
|
Platform ID |
GPL2995 |
Series (1) |
GSE39813 |
Gene expression changes in testes from Abcg8 knockout mice compared to wild-type mice or to Abcg8 knockout mice where plant sterol entry was blocked by ezetimibe |
|