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Sample GSM979675 Query DataSets for GSM979675
Status Public on Aug 02, 2012
Title Abcg8 knockout testes, 4573
Sample type RNA
 
Source name testes, Abcg8 knockout
Organism Mus musculus
Characteristics strain/background: C57BL/6J
genotype: Abcg8 knockout
gender: male
developmental stage: adult
age: 10-12 weeks
tissue: testes
Growth protocol Mice were fed rodent chow. All mice were between 10-12 weeks of age.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted and purified using RiboPure RNA Isolation (Ambion). Total RNA samples were quantitated by UV spectrophotometry. Quality of total RNA was assessed using an Agilent Bioanalyzer.
Label Digoxigenin
Label protocol First and second strand cDNA was prepared from the total RNA samples. Digoxigenin-labeled cRNA target was prepared from the DNA template and verified on the Bioanalyzer.
 
Hybridization protocol cRNA was fragmented to uniform size and verified on the Bioanalyzer. Applied Biosystems Mouse Genome Survey microarrays were hybridized with the cRNA target and treated for chemiluminescence detection.
Scan protocol Arrays were imaged and processed on the AB1700 Microarray Analyzer.
Description 4573
Data processing Data was analyzed with GeneSpring GX software. Intensity values were normalized to the median intensity of each array.
 
Submission date Aug 01, 2012
Last update date Aug 02, 2012
Contact name Michael Falduto
E-mail(s) mfalduto@genusbiosystems.com
Phone 847-291-9602
Organization name GenUs BioSystems, Inc.
Street address 1808 Janke, Unit M
City Northbrook
State/province IL
ZIP/Postal code 60062
Country USA
 
Platform ID GPL2995
Series (1)
GSE39813 Gene expression changes in testes from Abcg8 knockout mice compared to wild-type mice or to Abcg8 knockout mice where plant sterol entry was blocked by ezetimibe

Data table header descriptions
ID_REF
VALUE Normalized intensity value

Data table
ID_REF VALUE
297784 27.45
297907 0.33
297912 2.17
297935 266.71
297990 0.73
297993 65.61
298000 16.83
298038 0.30
298121 2.40
298130 0.36
298143 0.26
298150 0.18
298151 0.62
298155 0.44
298165 1.08
298174 9.33
298188 0.41
298200 159.96
298246 0.78
298248 24.46

Total number of rows: 33012

Table truncated, full table size 395 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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