|
Status |
Public on Jan 15, 2013 |
Title |
Unamputated wildtype adult zebrafish, sample 2 |
Sample type |
RNA |
|
|
Source name |
unamputated wildtype adult zebrafish
|
Organism |
Danio rerio |
Characteristics |
line: AB genotype: wildtype tissue: heart developmental stage: adult, sexually mature treatment: none
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
200 ng of total RNA were amplified and labeled using the WT Sense Target labeling system and control reagents (Affymetrix).
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|
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Hybridization protocol |
Samples were hybridized to GeneChip Zebrafish Genome Arrays (Affymetrix) using a GeneChip Hybridization Oven 640.
|
Scan protocol |
GeneChip was scanned using Affymetrix GeneChip Scanner 7G.
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Description |
WT2_0 Gene expression data from unamputated wildtype adult zebrafish hearts.
|
Data processing |
The data were analyzed using the RMA algorithm. Then, LIMMA was applied to calculate significant differential expression between samples.
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|
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Submission date |
Aug 02, 2012 |
Last update date |
Jan 15, 2013 |
Contact name |
Chris Jopling |
E-mail(s) |
chris.jopling@igf.cnrs.fr
|
Phone |
+33 434359253
|
Organization name |
Institut de Génomique Fonctionnelle
|
Department |
Physiologie
|
Lab |
Molecular mechansims of regeneration
|
Street address |
141 rue de la Cardonille
|
City |
Montpellier |
ZIP/Postal code |
34094 |
Country |
France |
|
|
Platform ID |
GPL1319 |
Series (1) |
GSE39842 |
Hypoxia induces myocardial regeneration in zebrafish |
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