|
| Status |
Public on Jan 15, 2013 |
| Title |
7 days post-amputation wildtype adult zebrafish, sample 1 |
| Sample type |
RNA |
| |
|
| Source name |
7 days post-amputation wildtype adult zebrafish
|
| Organism |
Danio rerio |
| Characteristics |
line: AB
genotype: wildtype
tissue: heart
developmental stage: adult, sexually mature
treatment: amputation
time post-treatment: 7 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
200 ng of total RNA were amplified and labeled using the WT Sense Target labeling system and control reagents (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized to GeneChip Zebrafish Genome Arrays (Affymetrix) using a GeneChip Hybridization Oven 640.
|
| Scan protocol |
GeneChip was scanned using Affymetrix GeneChip Scanner 7G.
|
| Description |
WT1_7
Gene expression data from 7 day post-amputation wildtype adult zebrafish hearts.
|
| Data processing |
The data were analyzed using the RMA algorithm. Then, LIMMA was applied to calculate significant differential expression between samples.
|
| |
|
| Submission date |
Aug 02, 2012 |
| Last update date |
Jan 15, 2013 |
| Contact name |
Chris Jopling |
| E-mail(s) |
chris.jopling@igf.cnrs.fr
|
| Phone |
+33 434359253
|
| Organization name |
Institut de Génomique Fonctionnelle
|
| Department |
Physiologie
|
| Lab |
Molecular mechansims of regeneration
|
| Street address |
141 rue de la Cardonille
|
| City |
Montpellier |
| ZIP/Postal code |
34094 |
| Country |
France |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE39842 |
Hypoxia induces myocardial regeneration in zebrafish |
|
