|
| Status |
Public on Aug 28, 2012 |
| Title |
WT_1 |
| Sample type |
RNA |
| |
|
| Source name |
Spleen and lymph nodes
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: WT
cell type: Tregs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Sorted Gata3 sufficient Tregs
|
| Data processing |
Robust Multi-array Average (RMA) analysis.
|
| |
|
| Submission date |
Aug 03, 2012 |
| Last update date |
Aug 28, 2012 |
| Contact name |
Aaron Arvey |
| E-mail(s) |
aarvey@cbio.mskcc.org
|
| Organization name |
Memorial Sloan-Kettering Cancer Center
|
| Street address |
415 E 68th St ZRC 1441
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE39864 |
Treg specific Gata3 knock out array |
|
