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Sample GSM980507 Query DataSets for GSM980507
Status Public on Aug 28, 2012
Title WT_3
Sample type RNA
 
Source name Spleen and lymph nodes
Organism Mus musculus
Characteristics genotype/variation: WT
cell type: Tregs
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Sorted Gata3 sufficient Tregs
Data processing Robust Multi-array Average (RMA) analysis.
 
Submission date Aug 03, 2012
Last update date Aug 28, 2012
Contact name Aaron Arvey
E-mail(s) aarvey@cbio.mskcc.org
Organization name Memorial Sloan-Kettering Cancer Center
Street address 415 E 68th St ZRC 1441
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platform ID GPL8321
Series (1)
GSE39864 Treg specific Gata3 knock out array

Data table header descriptions
ID_REF
VALUE RMA calculated signal intensity.

Data table
ID_REF VALUE
1415670_at 9.015404501
1415671_at 8.792667033
1415672_at 10.2649623
1415673_at 8.379694077
1415674_a_at 8.952077281
1415675_at 8.758333543
1415676_a_at 9.645154285
1415677_at 6.798761251
1415678_at 9.217483077
1415679_at 11.04362858
1415680_at 8.482430338
1415681_at 8.685871485
1415682_at 7.477595783
1415683_at 10.2669184
1415684_at 7.978212117
1415685_at 8.945429022
1415686_at 8.609954227
1415687_a_at 9.7536726
1415688_at 9.617526352
1415689_s_at 9.031895216

Total number of rows: 22690

Table truncated, full table size 521 Kbytes.




Supplementary file Size Download File type/resource
GSM980507_DR3_Mouse430A_2_WT3.CEL.gz 1.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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