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Sample GSM980508 Query DataSets for GSM980508
Status Public on Aug 28, 2012
Title GATA3KO_1
Sample type RNA
 
Source name Spleen and lymph nodes
Organism Mus musculus
Characteristics genotype/variation: GATA3 KO
cell type: Tregs
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Sorted Gata3 deficient Tregs
Data processing Robust Multi-array Average (RMA) analysis.
 
Submission date Aug 03, 2012
Last update date Aug 28, 2012
Contact name Aaron Arvey
E-mail(s) aarvey@cbio.mskcc.org
Organization name Memorial Sloan-Kettering Cancer Center
Street address 415 E 68th St ZRC 1441
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platform ID GPL8321
Series (1)
GSE39864 Treg specific Gata3 knock out array

Data table header descriptions
ID_REF
VALUE RMA calculated signal intensity.

Data table
ID_REF VALUE
1415670_at 9.177886288
1415671_at 8.716275548
1415672_at 10.30465057
1415673_at 8.209019438
1415674_a_at 9.021082768
1415675_at 8.755283922
1415676_a_at 9.518328018
1415677_at 6.885282109
1415678_at 9.188370969
1415679_at 10.95628335
1415680_at 8.484747606
1415681_at 8.642104909
1415682_at 7.361692159
1415683_at 10.31551906
1415684_at 7.935792658
1415685_at 9.156312445
1415686_at 8.579853801
1415687_a_at 9.733478979
1415688_at 9.497175758
1415689_s_at 8.85045194

Total number of rows: 22690

Table truncated, full table size 521 Kbytes.




Supplementary file Size Download File type/resource
GSM980508_DR4_Mouse430A_2_Gata3KO1.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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