|
Status |
Public on Aug 28, 2012 |
Title |
GATA3KO_1 |
Sample type |
RNA |
|
|
Source name |
Spleen and lymph nodes
|
Organism |
Mus musculus |
Characteristics |
genotype/variation: GATA3 KO cell type: Tregs
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
Description |
Sorted Gata3 deficient Tregs
|
Data processing |
Robust Multi-array Average (RMA) analysis.
|
|
|
Submission date |
Aug 03, 2012 |
Last update date |
Aug 28, 2012 |
Contact name |
Aaron Arvey |
E-mail(s) |
aarvey@cbio.mskcc.org
|
Organization name |
Memorial Sloan-Kettering Cancer Center
|
Street address |
415 E 68th St ZRC 1441
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10065 |
Country |
USA |
|
|
Platform ID |
GPL8321 |
Series (1) |
GSE39864 |
Treg specific Gata3 knock out array |
|