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Sample GSM980776 Query DataSets for GSM980776
Status Public on Oct 30, 2013
Title apoE-Spleen-32-weeks-2
Sample type RNA
Source name spleen
Organism Mus musculus
Characteristics strain: B6.129P2-Apoe<tm1Unc>/J
gender: male
age: 32 weeks
tissue: spleen
Treatment protocol excised, rinsed with cold PBS (2mM EDTA)
Growth protocol Maintained under normal mouse chow / SPF conditions (12h light-dark cycle)
Extracted molecule total RNA
Extraction protocol Qiagen RNEasy Lipid Tissue Mini Kit, Standardprotocol
Label Biotin/SAPE with Anti Streptavidin signal amplification
Label protocol ENZO T7 High Yield Transcript Labelling Kit, 24h IVT
Hybridization protocol 60rpm, 18h, 45 centigrade in Affymetrix Hybridization Buffer
Scan protocol Affymetrix Scanner 3000, single scan
Description Staining with Fluidic Station 400 Protocol: EukGe-WS2v4
Data processing GCOS 1.4, scaled to 500
Submission date Aug 06, 2012
Last update date Oct 31, 2013
Contact name Andreas Johann Richard Habenicht
Organization name Ludwig Maximilians Universität München
Department Medicine
Lab Institute for Cardiovascular Disease Prevention
Street address Pettenkoferstraße 9a
City Munich
State/province Bavaria
ZIP/Postal code 80336
Country Germany
Platform ID GPL1261
Series (1)
GSE40156 Transcript atlases reveal that artery tertiary lymphoid organs but not secondary lymphoid organs control key steps of atherosclerosis T cell immunity in aged apoe-/- mice.

Data table header descriptions
VALUE Signal value scaled to 500 by GCOS
ABS_CALL Call by default alpha
DETECTION P-VALUE Detection p-value as calculated by GCOS

Data table
AFFX-BioB-5_at 364.5 P 0.010317
AFFX-BioB-M_at 466.1 P 0.000127
AFFX-BioB-3_at 210 P 0.021902
AFFX-BioC-5_at 722.8 P 0.000297
AFFX-BioC-3_at 655.3 P 0.000297
AFFX-BioDn-5_at 905.5 P 0.00011
AFFX-BioDn-3_at 4658 P 0.000297
AFFX-CreX-5_at 12581.9 P 0.000052
AFFX-CreX-3_at 12895.7 P 0.000044
AFFX-DapX-5_at 589.7 P 0.00011
AFFX-DapX-M_at 1106.4 P 0.002556
AFFX-DapX-3_at 1016.5 P 0.000095
AFFX-LysX-5_at 77.3 P 0.00844
AFFX-LysX-M_at 159.9 A 0.354453
AFFX-LysX-3_at 267.3 P 0.000258
AFFX-PheX-5_at 58.1 A 0.262827
AFFX-PheX-M_at 79.4 A 0.089478
AFFX-PheX-3_at 152.1 A 0.095667
AFFX-ThrX-5_at 133.9 A 0.067678
AFFX-ThrX-M_at 162.6 P 0.006871

Total number of rows: 45101

Table truncated, full table size 1225 Kbytes.

Supplementary file Size Download File type/resource
GSM980776_apoE_Spleen_32_weeks_2.CEL.gz 4.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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