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Sample GSM980778 Query DataSets for GSM980778
Status Public on Oct 30, 2013
Title apoE-Spleen-78-weeks-1
Sample type RNA
 
Source name spleen
Organism Mus musculus
Characteristics strain: B6.129P2-Apoe<tm1Unc>/J
gender: male
age: 78 weeks
tissue: spleen
Treatment protocol excised, rinsed with cold PBS (2mM EDTA)
Growth protocol Maintained under normal mouse chow / SPF conditions (12h light-dark cycle)
Extracted molecule total RNA
Extraction protocol Qiagen RNEasy Lipid Tissue Mini Kit, Standardprotocol
Label Biotin/SAPE with Anti Streptavidin signal amplification
Label protocol ENZO T7 High Yield Transcript Labelling Kit, 24h IVT
 
Hybridization protocol 60rpm, 18h, 45 centigrade in Affymetrix Hybridization Buffer
Scan protocol Affymetrix Scanner 3000, single scan
Description Staining with Fluidic Station 400 Protocol: EukGe-WS2v4
Data processing GCOS 1.4, scaled to 500
 
Submission date Aug 06, 2012
Last update date Oct 31, 2013
Contact name Andreas Johann Richard Habenicht
E-mail(s) Andreas.habenicht@med.uni-muenchen.de
Organization name Ludwig Maximilians Universität München
Department Medicine
Lab Institute for Cardiovascular Disease Prevention
Street address Pettenkoferstraße 9a
City Munich
State/province Bavaria
ZIP/Postal code 80336
Country Germany
 
Platform ID GPL1261
Series (1)
GSE40156 Transcript atlases reveal that artery tertiary lymphoid organs but not secondary lymphoid organs control key steps of atherosclerosis T cell immunity in aged apoe-/- mice.

Data table header descriptions
ID_REF
VALUE Signal value scaled to 500 by GCOS
ABS_CALL Call by default alpha
DETECTION P-VALUE Detection p-value as calculated by GCOS

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 182.4 P 0.002556
AFFX-BioB-M_at 344.4 P 0.00007
AFFX-BioB-3_at 191.2 P 0.00039
AFFX-BioC-5_at 813.9 P 0.000147
AFFX-BioC-3_at 1029.7 P 0.000044
AFFX-BioDn-5_at 1758.7 P 0.00006
AFFX-BioDn-3_at 3843.5 P 0.000258
AFFX-CreX-5_at 10084.6 P 0.00006
AFFX-CreX-3_at 9739.2 P 0.000044
AFFX-DapX-5_at 357.8 P 0.00011
AFFX-DapX-M_at 655.2 P 0.002275
AFFX-DapX-3_at 572.4 P 0.000127
AFFX-LysX-5_at 29.4 A 0.0727
AFFX-LysX-M_at 120.7 A 0.239063
AFFX-LysX-3_at 128.6 P 0.000195
AFFX-PheX-5_at 27.6 A 0.425962
AFFX-PheX-M_at 63.6 P 0.026111
AFFX-PheX-3_at 60.4 A 0.239063
AFFX-ThrX-5_at 49.7 A 0.262827
AFFX-ThrX-M_at 131.7 P 0.006187

Total number of rows: 45101

Table truncated, full table size 1220 Kbytes.




Supplementary file Size Download File type/resource
GSM980778_apoE_Spleen_78_weeks_1.CEL.gz 4.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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