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Sample GSM980782 Query DataSets for GSM980782
Status Public on Oct 30, 2013
Title wt-Spleen-32-weeks-2
Sample type RNA
 
Source name spleen
Organism Mus musculus
Characteristics strain: C57BL/6J
gender: male
age: 32 weeks
tissue: spleen
Treatment protocol excised, rinsed with cold PBS (2mM EDTA)
Growth protocol Maintained under normal mouse chow / SPF conditions (12h light-dark cycle)
Extracted molecule total RNA
Extraction protocol Qiagen RNEasy Lipid Tissue Mini Kit, Standardprotocol
Label Biotin/SAPE with Anti Streptavidin signal amplification
Label protocol ENZO T7 High Yield Transcript Labelling Kit, 24h IVT
 
Hybridization protocol 60rpm, 18h, 45 centigrade in Affymetrix Hybridization Buffer
Scan protocol Affymetrix Scanner 3000, single scan
Description Staining with Fluidic Station 400 Protocol: EukGe-WS2v4
Data processing GCOS 1.4, scaled to 500
 
Submission date Aug 06, 2012
Last update date Oct 31, 2013
Contact name Andreas Johann Richard Habenicht
E-mail(s) Andreas.habenicht@med.uni-muenchen.de
Organization name Ludwig Maximilians Universität München
Department Medicine
Lab Institute for Cardiovascular Disease Prevention
Street address Pettenkoferstraße 9a
City Munich
State/province Bavaria
ZIP/Postal code 80336
Country Germany
 
Platform ID GPL1261
Series (1)
GSE40156 Transcript atlases reveal that artery tertiary lymphoid organs but not secondary lymphoid organs control key steps of atherosclerosis T cell immunity in aged apoe-/- mice.

Data table header descriptions
ID_REF
VALUE Signal value scaled to 500 by GCOS
ABS_CALL Call by default alpha
DETECTION P-VALUE Detection p-value as calculated by GCOS

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 397.2 P 0.001796
AFFX-BioB-M_at 981.3 P 0.000052
AFFX-BioB-3_at 474.8 P 0.000972
AFFX-BioC-5_at 1166.2 P 0.000169
AFFX-BioC-3_at 1039.2 P 0.00011
AFFX-BioDn-5_at 1354.1 P 0.00006
AFFX-BioDn-3_at 6365 P 0.000095
AFFX-CreX-5_at 16833.8 P 0.000052
AFFX-CreX-3_at 18480.1 P 0.000044
AFFX-DapX-5_at 715.8 P 0.000127
AFFX-DapX-M_at 974.1 P 0.001248
AFFX-DapX-3_at 1130.6 P 0.00007
AFFX-LysX-5_at 68.5 P 0.006187
AFFX-LysX-M_at 126.6 A 0.287743
AFFX-LysX-3_at 259 P 0.000297
AFFX-PheX-5_at 69.2 A 0.102165
AFFX-PheX-M_at 100.9 A 0.078002
AFFX-PheX-3_at 125.5 A 0.067678
AFFX-ThrX-5_at 170.9 P 0.033677
AFFX-ThrX-M_at 214.9 P 0.001593

Total number of rows: 45101

Table truncated, full table size 1224 Kbytes.




Supplementary file Size Download File type/resource
GSM980782_wt_Spleen_32_weeks_2.CEL.gz 4.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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