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Sample GSM980785 Query DataSets for GSM980785
Status Public on Oct 30, 2013
Title wt-Spleen-78-weeks-2
Sample type RNA
 
Source name spleen
Organism Mus musculus
Characteristics strain: C57BL/6J
gender: male
age: 78 weeks
tissue: spleen
Treatment protocol excised, rinsed with cold PBS (2mM EDTA)
Growth protocol Maintained under normal mouse chow / SPF conditions (12h light-dark cycle)
Extracted molecule total RNA
Extraction protocol Qiagen RNEasy Lipid Tissue Mini Kit, Standardprotocol
Label Biotin/SAPE with Anti Streptavidin signal amplification
Label protocol ENZO T7 High Yield Transcript Labelling Kit, 24h IVT
 
Hybridization protocol 60rpm, 18h, 45 centigrade in Affymetrix Hybridization Buffer
Scan protocol Affymetrix Scanner 3000, single scan
Description Staining with Fluidic Station 400 Protocol: EukGe-WS2v4
Data processing GCOS 1.4, scaled to 500
 
Submission date Aug 06, 2012
Last update date Oct 31, 2013
Contact name Andreas Johann Richard Habenicht
E-mail(s) Andreas.habenicht@med.uni-muenchen.de
Organization name Ludwig Maximilians Universität München
Department Medicine
Lab Institute for Cardiovascular Disease Prevention
Street address Pettenkoferstraße 9a
City Munich
State/province Bavaria
ZIP/Postal code 80336
Country Germany
 
Platform ID GPL1261
Series (1)
GSE40156 Transcript atlases reveal that artery tertiary lymphoid organs but not secondary lymphoid organs control key steps of atherosclerosis T cell immunity in aged apoe-/- mice.

Data table header descriptions
ID_REF
VALUE Signal value scaled to 500 by GCOS
ABS_CALL Call by default alpha
DETECTION P-VALUE Detection p-value as calculated by GCOS

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 297.6 P 0.003595
AFFX-BioB-M_at 332.8 P 0.000195
AFFX-BioB-3_at 217.2 P 0.000857
AFFX-BioC-5_at 817.7 P 0.000147
AFFX-BioC-3_at 924.5 P 0.000052
AFFX-BioDn-5_at 1665.9 P 0.00007
AFFX-BioDn-3_at 3848.4 P 0.000225
AFFX-CreX-5_at 9507.5 P 0.00007
AFFX-CreX-3_at 10470.5 P 0.000044
AFFX-DapX-5_at 309.1 P 0.000127
AFFX-DapX-M_at 625.4 P 0.002275
AFFX-DapX-3_at 514.1 P 0.000127
AFFX-LysX-5_at 33 M 0.050229
AFFX-LysX-M_at 89.9 A 0.287743
AFFX-LysX-3_at 133 P 0.000225
AFFX-PheX-5_at 47.9 A 0.275146
AFFX-PheX-M_at 68 A 0.156732
AFFX-PheX-3_at 55.4 A 0.205712
AFFX-ThrX-5_at 44.9 A 0.275146
AFFX-ThrX-M_at 106 P 0.033677

Total number of rows: 45101

Table truncated, full table size 1222 Kbytes.




Supplementary file Size Download File type/resource
GSM980785_wt_Spleen_78_weeks_2.CEL.gz 4.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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