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Sample GSM980821 Query DataSets for GSM980821
Status Public on Oct 30, 2013
Title apoE-Blood-78-weeks-2
Sample type RNA
 
Source name blood
Organism Mus musculus
Characteristics strain: B6.129P2-Apoe<tm1Unc>/J
gender: male
age: 78 weeks
tissue: blood
Treatment protocol Mouse blood was drawn by heart puncture into EDTA buffer, erythrocytes were lyzed, total blood cells were centrifuged, and taken up in Trizol.
Growth protocol Maintained under normal mouse chow / SPF conditions (12h light-dark cycle)
Extracted molecule total RNA
Extraction protocol Qiagen RNEasy Lipid Tissue Mini Kit, Standardprotocol
Label Biotin/SAPE with Anti Streptavidin signal amplification
Label protocol ENZO T7 High Yield Transcript Labelling Kit, 24h IVT
 
Hybridization protocol 60rpm, 18h, 45 centigrade in Affymetrix Hybridization Buffer
Scan protocol Affymetrix Scanner 3000, single scan
Description Staining with Fluidic Station 400 Protocol: EukGe-WS2v4
Data processing GCOS 1.4, scaled to 500
 
Submission date Aug 06, 2012
Last update date Oct 31, 2013
Contact name Andreas Johann Richard Habenicht
E-mail(s) Andreas.habenicht@med.uni-muenchen.de
Organization name Ludwig Maximilians Universität München
Department Medicine
Lab Institute for Cardiovascular Disease Prevention
Street address Pettenkoferstraße 9a
City Munich
State/province Bavaria
ZIP/Postal code 80336
Country Germany
 
Platform ID GPL1261
Series (1)
GSE40156 Transcript atlases reveal that artery tertiary lymphoid organs but not secondary lymphoid organs control key steps of atherosclerosis T cell immunity in aged apoe-/- mice.

Data table header descriptions
ID_REF
VALUE Signal value scaled to 500 by GCOS
ABS_CALL Call by default alpha
DETECTION P-VALUE Detection p-value as calculated by GCOS

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 322.7 P 0.011384
AFFX-BioB-M_at 432.4 P 0.000127
AFFX-BioB-3_at 292.5 P 0.001248
AFFX-BioC-5_at 905.4 P 0.00034
AFFX-BioC-3_at 1030.6 P 0.000052
AFFX-BioDn-5_at 1956.2 P 0.00006
AFFX-BioDn-3_at 4922.8 P 0.000446
AFFX-CreX-5_at 11245 P 0.00007
AFFX-CreX-3_at 11793.7 P 0.000044
AFFX-DapX-5_at 99.5 P 0.012547
AFFX-DapX-M_at 113.1 P 0.009337
AFFX-DapX-3_at 131.3 P 0.046467
AFFX-LysX-5_at 6.1 A 0.574038
AFFX-LysX-M_at 46.2 A 0.574038
AFFX-LysX-3_at 54.5 P 0.018281
AFFX-PheX-5_at 4.9 A 0.794268
AFFX-PheX-M_at 4.6 A 0.783476
AFFX-PheX-3_at 22 A 0.60308
AFFX-ThrX-5_at 21 A 0.52976
AFFX-ThrX-M_at 50.9 A 0.216524

Total number of rows: 45101

Table truncated, full table size 1221 Kbytes.




Supplementary file Size Download File type/resource
GSM980821_apoE_Blood_78_weeks_2.CEL.gz 4.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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