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Sample GSM980825 Query DataSets for GSM980825
Status Public on Oct 30, 2013
Title wt-Blood-32-weeks-3
Sample type RNA
Source name blood
Organism Mus musculus
Characteristics strain: C57BL/6J
gender: male
age: 32 weeks
tissue: blood
Treatment protocol Mouse blood was drawn by heart puncture into EDTA buffer, erythrocytes were lyzed, total blood cells were centrifuged, and taken up in Trizol.
Growth protocol Maintained under normal mouse chow / SPF conditions (12h light-dark cycle)
Extracted molecule total RNA
Extraction protocol Qiagen RNEasy Lipid Tissue Mini Kit, Standardprotocol
Label Biotin/SAPE with Anti Streptavidin signal amplification
Label protocol ENZO T7 High Yield Transcript Labelling Kit, 24h IVT
Hybridization protocol 60rpm, 18h, 45 centigrade in Affymetrix Hybridization Buffer
Scan protocol Affymetrix Scanner 3000, single scan
Description Staining with Fluidic Station 400 Protocol: EukGe-WS2v4
Data processing GCOS 1.4, scaled to 500
Submission date Aug 06, 2012
Last update date Oct 31, 2013
Contact name Andreas Johann Richard Habenicht
Organization name Ludwig Maximilians Universität München
Department Medicine
Lab Institute for Cardiovascular Disease Prevention
Street address Pettenkoferstraße 9a
City Munich
State/province Bavaria
ZIP/Postal code 80336
Country Germany
Platform ID GPL1261
Series (1)
GSE40156 Transcript atlases reveal that artery tertiary lymphoid organs but not secondary lymphoid organs control key steps of atherosclerosis T cell immunity in aged apoe-/- mice.

Data table header descriptions
VALUE Signal value scaled to 500 by GCOS
ABS_CALL Call by default alpha
DETECTION P-VALUE Detection p-value as calculated by GCOS

Data table
AFFX-BioB-5_at 341.6 P 0.004017
AFFX-BioB-M_at 634.1 P 0.000052
AFFX-BioB-3_at 321.9 P 0.000662
AFFX-BioC-5_at 991.5 P 0.000225
AFFX-BioC-3_at 822.2 P 0.000095
AFFX-BioDn-5_at 1169.5 P 0.00007
AFFX-BioDn-3_at 4801 P 0.000147
AFFX-CreX-5_at 14237.8 P 0.000052
AFFX-CreX-3_at 13910.4 P 0.000044
AFFX-DapX-5_at 718.6 P 0.000081
AFFX-DapX-M_at 1070 P 0.001796
AFFX-DapX-3_at 1136.4 P 0.00007
AFFX-LysX-5_at 63.4 P 0.001796
AFFX-LysX-M_at 143.7 A 0.239063
AFFX-LysX-3_at 259.2 P 0.00039
AFFX-PheX-5_at 99.9 A 0.095667
AFFX-PheX-M_at 94.3 P 0.028457
AFFX-PheX-3_at 138.9 M 0.050229
AFFX-ThrX-5_at 93.5 P 0.012547
AFFX-ThrX-M_at 194.5 P 0.010317

Total number of rows: 45101

Table truncated, full table size 1222 Kbytes.

Supplementary file Size Download File type/resource
GSM980825_wt_Blood_32_weeks_3.CEL.gz 4.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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