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Sample GSM980827 Query DataSets for GSM980827
Status Public on Oct 30, 2013
Title wt-Blood-78-weeks-2
Sample type RNA
 
Source name blood
Organism Mus musculus
Characteristics strain: C57BL/6J
gender: male
age: 78 weeks
tissue: blood
Treatment protocol Mouse blood was drawn by heart puncture into EDTA buffer, erythrocytes were lyzed, total blood cells were centrifuged, and taken up in Trizol.
Growth protocol Maintained under normal mouse chow / SPF conditions (12h light-dark cycle)
Extracted molecule total RNA
Extraction protocol Qiagen RNEasy Lipid Tissue Mini Kit, Standardprotocol
Label Biotin/SAPE with Anti Streptavidin signal amplification
Label protocol ENZO T7 High Yield Transcript Labelling Kit, 24h IVT
 
Hybridization protocol 60rpm, 18h, 45 centigrade in Affymetrix Hybridization Buffer
Scan protocol Affymetrix Scanner 3000, single scan
Description Staining with Fluidic Station 400 Protocol: EukGe-WS2v4
Data processing GCOS 1.4, scaled to 500
 
Submission date Aug 06, 2012
Last update date Oct 31, 2013
Contact name Andreas Johann Richard Habenicht
E-mail(s) Andreas.habenicht@med.uni-muenchen.de
Organization name Ludwig Maximilians Universität München
Department Medicine
Lab Institute for Cardiovascular Disease Prevention
Street address Pettenkoferstraße 9a
City Munich
State/province Bavaria
ZIP/Postal code 80336
Country Germany
 
Platform ID GPL1261
Series (1)
GSE40156 Transcript atlases reveal that artery tertiary lymphoid organs but not secondary lymphoid organs control key steps of atherosclerosis T cell immunity in aged apoe-/- mice.

Data table header descriptions
ID_REF
VALUE Signal value scaled to 500 by GCOS
ABS_CALL Call by default alpha
DETECTION P-VALUE Detection p-value as calculated by GCOS

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 342.8 P 0.001593
AFFX-BioB-M_at 599.5 P 0.00011
AFFX-BioB-3_at 365.8 P 0.000147
AFFX-BioC-5_at 1349.5 P 0.000081
AFFX-BioC-3_at 1603.9 P 0.000052
AFFX-BioDn-5_at 2528.7 P 0.000044
AFFX-BioDn-3_at 5632.6 P 0.00007
AFFX-CreX-5_at 12706 P 0.000052
AFFX-CreX-3_at 14020.5 P 0.000044
AFFX-DapX-5_at 399.4 P 0.000225
AFFX-DapX-M_at 644 P 0.001102
AFFX-DapX-3_at 542.4 P 0.00011
AFFX-LysX-5_at 63.3 P 0.011384
AFFX-LysX-M_at 85.5 A 0.327079
AFFX-LysX-3_at 160.2 P 0.000297
AFFX-PheX-5_at 72.8 A 0.108979
AFFX-PheX-M_at 82.5 P 0.009337
AFFX-PheX-3_at 102.4 P 0.046482
AFFX-ThrX-5_at 71.8 M 0.054213
AFFX-ThrX-M_at 143.7 P 0.00762

Total number of rows: 45101

Table truncated, full table size 1220 Kbytes.




Supplementary file Size Download File type/resource
GSM980827_wt_Blood_78_weeks_2.CEL.gz 4.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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