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Sample GSM983011 Query DataSets for GSM983011
Status Public on Aug 08, 2013
Title tAML patient sample SJTAML002
Sample type RNA
 
Source name RNA extracted from tumor cells
Organism Homo sapiens
Characteristics disease state: therapy-related myeloid neoplasm (t-MN)
chromosome 5q status: Chromosome 5q deletion
cell type: mononuclear cell
Treatment protocol None
Growth protocol RNA was extracted from cryopreserved mononuclear cell preparations of diagnostic bone marrow or peripheral blood from t-MN cases
Extracted molecule total RNA
Extraction protocol Trizol
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix 3’IVT Express protocol starting with 100 ng of total RNA (GeneChip 3’ IVT Express Kit 702646 Rev. 8, 2008-2010, Affymetrix)
 
Hybridization protocol Following fragmentation, 5 microgram of cRNA was hybridized for 16 hr at 45C on each array. GeneChips were washed and stained in the Affymetrix GeneTitan system.
Scan protocol GeneChips were scanned using the Affymetrix GeneTitan system.
Data processing Data were background adjusted, normalized and summarized by the RMA method. The Bioconductor package was used for this analysis.
 
Submission date Aug 08, 2012
Last update date Aug 08, 2013
Contact name Shann-Ching Chen
E-mail(s) Shann-Ching.Chen@stjude.org
Organization name St. Jude Children's Research Hospital
Department Pathology
Street address 262 Danny Thomas Place
City Memphis
State/province TN
ZIP/Postal code 38105
Country USA
 
Platform ID GPL13158
Series (1)
GSE39991 Knockdown of Hnrnpa0, a del(5q) Gene, Alters Myeloid Cell Fate in Murine Cells through Regulation of AU-rich Transcripts

Data table header descriptions
ID_REF
VALUE RMA signal intensity (log2 scale)

Data table
ID_REF VALUE
1007_PM_s_at 3.756319476
1053_PM_at 5.756180518
117_PM_at 5.90263007
121_PM_at 4.4125555
1255_PM_g_at 1.827481332
1294_PM_at 4.971722548
1316_PM_at 3.676901293
1320_PM_at 2.423499437
1405_PM_i_at 7.103856166
1431_PM_at 2.282287079
1438_PM_at 3.046287306
1487_PM_at 4.24751573
1494_PM_f_at 2.409320095
1552256_PM_a_at 5.417227463
1552257_PM_a_at 6.051863786
1552258_PM_at 3.309493651
1552261_PM_at 2.378630511
1552263_PM_at 6.888634393
1552264_PM_a_at 7.064480973
1552266_PM_at 2.039375596

Total number of rows: 54715

Table truncated, full table size 1373 Kbytes.




Supplementary file Size Download File type/resource
GSM983011_SJTAML002_HTU133PM.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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