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Sample GSM983020 Query DataSets for GSM983020
Status Public on Aug 08, 2013
Title tAML patient sample SJTAML028
Sample type RNA
 
Source name RNA extracted from tumor cells
Organism Homo sapiens
Characteristics disease state: therapy-related myeloid neoplasm (t-MN)
chromosome 5q status: Chromosome 5q deletion
cell type: mononuclear cell
Treatment protocol None
Growth protocol RNA was extracted from cryopreserved mononuclear cell preparations of diagnostic bone marrow or peripheral blood from t-MN cases
Extracted molecule total RNA
Extraction protocol Trizol
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix 3’IVT Express protocol starting with 100 ng of total RNA (GeneChip 3’ IVT Express Kit 702646 Rev. 8, 2008-2010, Affymetrix)
 
Hybridization protocol Following fragmentation, 5 microgram of cRNA was hybridized for 16 hr at 45C on each array. GeneChips were washed and stained in the Affymetrix GeneTitan system.
Scan protocol GeneChips were scanned using the Affymetrix GeneTitan system.
Data processing Data were background adjusted, normalized and summarized by the RMA method. The Bioconductor package was used for this analysis.
 
Submission date Aug 08, 2012
Last update date Aug 08, 2013
Contact name Shann-Ching Chen
E-mail(s) Shann-Ching.Chen@stjude.org
Organization name St. Jude Children's Research Hospital
Department Pathology
Street address 262 Danny Thomas Place
City Memphis
State/province TN
ZIP/Postal code 38105
Country USA
 
Platform ID GPL13158
Series (1)
GSE39991 Knockdown of Hnrnpa0, a del(5q) Gene, Alters Myeloid Cell Fate in Murine Cells through Regulation of AU-rich Transcripts

Data table header descriptions
ID_REF
VALUE RMA signal intensity (log2 scale)

Data table
ID_REF VALUE
1007_PM_s_at 2.833753327
1053_PM_at 5.387725421
117_PM_at 3.008712404
121_PM_at 4.505835906
1255_PM_g_at 2.005489227
1294_PM_at 6.805925154
1316_PM_at 3.575611411
1320_PM_at 2.394758962
1405_PM_i_at 5.125214236
1431_PM_at 2.091684864
1438_PM_at 2.303913739
1487_PM_at 4.773887078
1494_PM_f_at 2.616624616
1552256_PM_a_at 4.655761475
1552257_PM_a_at 6.609402614
1552258_PM_at 3.381358299
1552261_PM_at 2.315121178
1552263_PM_at 7.658638537
1552264_PM_a_at 7.142596407
1552266_PM_at 2.119040657

Total number of rows: 54715

Table truncated, full table size 1373 Kbytes.




Supplementary file Size Download File type/resource
GSM983020_SJTAML028_HTU133PM.CEL.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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