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Sample GSM983031 Query DataSets for GSM983031
Status Public on Aug 08, 2013
Title tAML patient sample SJTAML049
Sample type RNA
 
Source name RNA extracted from tumor cells
Organism Homo sapiens
Characteristics disease state: therapy-related myeloid neoplasm (t-MN)
chromosome 5q status: Chromosome 5q wild type
cell type: mononuclear cell
Treatment protocol None
Growth protocol RNA was extracted from cryopreserved mononuclear cell preparations of diagnostic bone marrow or peripheral blood from t-MN cases
Extracted molecule total RNA
Extraction protocol Trizol
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix 3’IVT Express protocol starting with 100 ng of total RNA (GeneChip 3’ IVT Express Kit 702646 Rev. 8, 2008-2010, Affymetrix)
 
Hybridization protocol Following fragmentation, 5 microgram of cRNA was hybridized for 16 hr at 45C on each array. GeneChips were washed and stained in the Affymetrix GeneTitan system.
Scan protocol GeneChips were scanned using the Affymetrix GeneTitan system.
Data processing Data were background adjusted, normalized and summarized by the RMA method. The Bioconductor package was used for this analysis.
 
Submission date Aug 08, 2012
Last update date Aug 08, 2013
Contact name Shann-Ching Chen
E-mail(s) Shann-Ching.Chen@stjude.org
Organization name St. Jude Children's Research Hospital
Department Pathology
Street address 262 Danny Thomas Place
City Memphis
State/province TN
ZIP/Postal code 38105
Country USA
 
Platform ID GPL13158
Series (1)
GSE39991 Knockdown of Hnrnpa0, a del(5q) Gene, Alters Myeloid Cell Fate in Murine Cells through Regulation of AU-rich Transcripts

Data table header descriptions
ID_REF
VALUE RMA signal intensity (log2 scale)

Data table
ID_REF VALUE
1007_PM_s_at 3.909404993
1053_PM_at 5.090222697
117_PM_at 3.992957876
121_PM_at 3.850696406
1255_PM_g_at 1.694831851
1294_PM_at 7.883298337
1316_PM_at 3.948592736
1320_PM_at 2.274583101
1405_PM_i_at 9.124790908
1431_PM_at 2.349216766
1438_PM_at 2.242352436
1487_PM_at 3.955254124
1494_PM_f_at 2.129385045
1552256_PM_a_at 5.672701711
1552257_PM_a_at 6.22664297
1552258_PM_at 3.079076311
1552261_PM_at 2.273925898
1552263_PM_at 6.49714892
1552264_PM_a_at 5.835821377
1552266_PM_at 2.480498351

Total number of rows: 54715

Table truncated, full table size 1373 Kbytes.




Supplementary file Size Download File type/resource
GSM983031_SJTAML049_HTU133PM.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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