Total RNA was isolated from liver samples by using TRIzol reagent (Invitrogen, Merelbeke, Belgium) according to manufacturer's instructions.
Label
biotin
Label protocol
Labelling of the samples was carried out at the Microarray Facility in Leuven (MAF, Leuven, Belgium).
Hybridization protocol
Hydridizations and washing of the chips were carried out at the Microarray Facility in Leuven (MAF, Leuven, Belgium).
Scan protocol
Scanning of the chips was carried out at the Microarray Facility in Leuven (MAF, Leuven, Belgium).
Description
knock-out 4
Data processing
The data were collected using the Affymetrix standard protocol and processed and normalized using Chipster Software Suite version 2.0 using Affymetrix default analysis settings and RMA as normalization method. Variances were not stabilized.