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Sample GSM98549 Query DataSets for GSM98549
Status Public on Mar 20, 2006
Title single cell from ICM E3.5, biological rep2
Sample type RNA
Source name Single cell from ICM in blastocyst at E3.5
Organism Mus musculus
Characteristics Strain: C57BL/6
Extracted molecule total RNA
Extraction protocol Single cell was lysed by adding to the tube containing cell lysis buffer, and the clude lysate was used for cDNA synthesis and amplification without purification.
Label biotin
Label protocol Biotinylated cRNA were prepared from the PCR-amplified double-starnded cDNA according to the standard Affymetrix protocol (Affymetrix GeneChip Expression Analysis Technical Manual, 2004, Affymetrix: Eukaryotic One-Cycle Target Labeling Assay)
Hybridization protocol Hybridization was performed according to the standard Affymetrix protocol (Affymetrix GeneChip Expression Analysis Technical Manual, 2004, Affymetrix: Eukaryotic Target Hybridization)
Scan protocol The microarray image data were processed with the GeneChip Scanner 3000 (Affymetrix)
Description Gene expression data from single ICM cells (E3.5)
Data processing CEL data were generated by GeneChip Operating Software and then subjected to the dCHIP software. Data obtained from the eight amplified samples (ES-10pg-amplified-rep1:8) were normalized together with the default settings. The Model-Based Expression Indices (MBEI) were calculated using the PM/MM difference mode with log-2 transformation of signal intensity and truncation of low values to zero.
Submission date Feb 27, 2006
Last update date Aug 28, 2018
Contact name Kazuki Kurimoto
Organization name Kyoto University
Department Graduate school of medicine
Lab Department of anatomy and cell biology
Street address Yoshida-Konoe-cho, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8501
Country Japan
Platform ID GPL1261
Series (2)
GSE4307 Expression data from single cells from ICMs of mouse blastocysts at E3.5
GSE4309 An improved single-cell cDNA amplification method for efficient high-density oligonucleotide microarray analysis
Reanalyzed by GSE119085

Data table header descriptions
VALUE Model-based Expression Index calculated with dCHIP
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)

Data table
AFFX-BioB-5_at 280.6328333 P
AFFX-BioB-M_at 344.4474291 P
AFFX-BioB-3_at 285.4907119 P
AFFX-BioC-5_at 573.3769108 P
AFFX-BioC-3_at 914.1011465 P
AFFX-BioDn-5_at 1861.435614 P
AFFX-BioDn-3_at 3110.218023 P
AFFX-CreX-5_at 6810.759625 P
AFFX-CreX-3_at 7640.228124 P
AFFX-DapX-5_at 34.69942858 A
AFFX-DapX-M_at 181.7561033 A
AFFX-DapX-3_at 854.0045819 P
AFFX-LysX-5_at 1971.666732 P
AFFX-LysX-M_at 3607.790875 P
AFFX-LysX-3_at 5658.484518 P
AFFX-PheX-5_at 97.58111074 P
AFFX-PheX-M_at 238.8696912 P
AFFX-PheX-3_at 228.5205346 P
AFFX-ThrX-5_at 49.08601015 P
AFFX-ThrX-M_at 46.41277329 P

Total number of rows: 45101

Table truncated, full table size 1067 Kbytes.

Supplementary file Size Download File type/resource
GSM98549.CEL.gz 5.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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