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Sample GSM98554 Query DataSets for GSM98554
Status Public on Mar 20, 2006
Title single cell from ICM E3.5, biological rep7
Sample type RNA
 
Source name Single cell from ICM in blastocyst at E3.5
Organism Mus musculus
Characteristics Strain: C57BL/6
Extracted molecule total RNA
Extraction protocol Single cell was lysed by adding to the tube containing cell lysis buffer, and the clude lysate was used for cDNA synthesis and amplification without purification.
Label biotin
Label protocol Biotinylated cRNA were prepared from the PCR-amplified double-starnded cDNA according to the standard Affymetrix protocol (Affymetrix GeneChip Expression Analysis Technical Manual, 2004, Affymetrix: Eukaryotic One-Cycle Target Labeling Assay)
 
Hybridization protocol Hybridization was performed according to the standard Affymetrix protocol (Affymetrix GeneChip Expression Analysis Technical Manual, 2004, Affymetrix: Eukaryotic Target Hybridization)
Scan protocol The microarray image data were processed with the GeneChip Scanner 3000 (Affymetrix)
Description Gene expression data from single ICM cells (E3.5)
Data processing CEL data were generated by GeneChip Operating Software and then subjected to the dCHIP software. Data obtained from the eight amplified samples (ES-10pg-amplified-rep1:8) were normalized together with the default settings. The Model-Based Expression Indices (MBEI) were calculated using the PM/MM difference mode with log-2 transformation of signal intensity and truncation of low values to zero.
 
Submission date Feb 27, 2006
Last update date Aug 28, 2018
Contact name Kazuki Kurimoto
E-mail(s) kurimoto@anat2.med.kyoto-u.ac.jp
Organization name Kyoto University
Department Graduate school of medicine
Lab Department of anatomy and cell biology
Street address Yoshida-Konoe-cho, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8501
Country Japan
 
Platform ID GPL1261
Series (2)
GSE4307 Expression data from single cells from ICMs of mouse blastocysts at E3.5
GSE4309 An improved single-cell cDNA amplification method for efficient high-density oligonucleotide microarray analysis
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Model-based Expression Index calculated with dCHIP
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)

Data table
ID_REF VALUE ABS_CALL
AFFX-BioB-5_at 247.8030269 P
AFFX-BioB-M_at 383.9940111 P
AFFX-BioB-3_at 301.7751081 P
AFFX-BioC-5_at 607.3860652 P
AFFX-BioC-3_at 875.5100929 P
AFFX-BioDn-5_at 1650.743214 P
AFFX-BioDn-3_at 2674.215475 P
AFFX-CreX-5_at 6685.871909 P
AFFX-CreX-3_at 7415.868774 P
AFFX-DapX-5_at 30.35852937 A
AFFX-DapX-M_at 344.0489427 P
AFFX-DapX-3_at 1138.248624 P
AFFX-LysX-5_at 2034.981551 P
AFFX-LysX-M_at 3544.336832 P
AFFX-LysX-3_at 6021.052577 P
AFFX-PheX-5_at 1 A
AFFX-PheX-M_at 119.9010099 P
AFFX-PheX-3_at 119.901841 P
AFFX-ThrX-5_at 19.13377986 A
AFFX-ThrX-M_at 59.91893183 A

Total number of rows: 45101

Table truncated, full table size 1067 Kbytes.




Supplementary file Size Download File type/resource
GSM98554.CEL.gz 5.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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