|
Status |
Public on Mar 20, 2006 |
Title |
ES 10pg amplified, technical rep1 |
Sample type |
RNA |
|
|
Source name |
Total RNA from Embryonic Stem cell culture diluted to 10 pg
|
Organism |
Mus musculus |
Characteristics |
Line: E14tg2a
|
Extracted molecule |
total RNA |
Extraction protocol |
The total RNA was prepared by the RNeasy Mini Kit (Qiagen) according to the manufacturer's instruction
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared from the PCR-amplified double-starnded cDNA according to the standard Affymetrix protocol (Affymetrix GeneChip Expression Analysis Technical Manual, 2004, Affymetrix: Eukaryotic One-Cycle Target Labeling Assay)
|
|
|
Hybridization protocol |
Hybridization was performed according to the standard Affymetrix protocol (Affymetrix GeneChip Expression Analysis Technical Manual, 2004, Affymetrix: Eukaryotic Target Hybridization)
|
Scan protocol |
The microarray image data were processed with the GeneChip Scanner 3000 (Affymetrix)
|
Description |
Gene expression data to verify the PCR-based single-cell cDNA amplification method (the V1V3 method)
|
Data processing |
CEL data were generated by GeneChip Operating Software and then subjected to the dCHIP software. Data obtained from the eight amplified samples (ES-10pg-amplified-rep1:8) were normalized together with the default settings. The Model-Based Expression Indices (MBEI) were calculated using the PM/MM difference mode with log-2 transformation of signal intensity and truncation of low values to zero.
|
|
|
Submission date |
Feb 27, 2006 |
Last update date |
Aug 28, 2018 |
Contact name |
Kazuki Kurimoto |
E-mail(s) |
kurimoto@naramed-u.ac.jp
|
Organization name |
Nara Medical University
|
Department |
School of medicine
|
Lab |
Department of Embryology
|
Street address |
840 Shijo-Cho, Kashihara
|
City |
Nara |
ZIP/Postal code |
634-8521 |
Country |
Japan |
|
|
Platform ID |
GPL1261 |
Series (2) |
GSE4308 |
Expression data for validation of single cell cDNA amplification method (V1V3 method) |
GSE4309 |
An improved single-cell cDNA amplification method for efficient high-density oligonucleotide microarray analysis |
|
Relations |
Reanalyzed by |
GSE119085 |