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Sample GSM98583 Query DataSets for GSM98583
Status Public on Mar 20, 2006
Title ES 5ug nonamplified, technical rep8
Sample type RNA
 
Source name Total RNA from Embryonic Stem cell culture (5ug)
Organism Mus musculus
Characteristics Line: E14tg2a
Extracted molecule total RNA
Extraction protocol The total RNA was prepared by the RNeasy Mini Kit (Qiagen) according to the manufacturer's instruction
Label biotin
Label protocol Biotinylated cRNA were prepared from total RNA from cultured ES cells according to the standard Affymetrix protocol (Affymetrix GeneChip Expression Analysis Technical Manual, 2004, Affymetrix: Eukaryotic One-Cycle Target Labeling Assay)
 
Hybridization protocol Hybridization was performed according to the standard Affymetrix protocol (Affymetrix GeneChip Expression Analysis Technical Manual, 2004, Affymetrix: Eukaryotic Target Hybridization)
Scan protocol The microarray image data were processed with the GeneChip Scanner 3000 (Affymetrix)
Description Gene expression data to verify the PCR-based single-cell cDNA amplification method (the V1V3 method)
Data processing CEL data were generated by GeneChip Operating Software and then subjected to the dCHIP software. Data obtained from the eight amplified samples (ES-10pg-amplified-rep1:8) were normalized together with the default settings. The Model-Based Expression Indices (MBEI) were calculated using the PM/MM difference mode with log-2 transformation of signal intensity and truncation of low values to zero.
 
Submission date Feb 27, 2006
Last update date Aug 28, 2018
Contact name Kazuki Kurimoto
E-mail(s) kurimoto@anat2.med.kyoto-u.ac.jp
Organization name Kyoto University
Department Graduate school of medicine
Lab Department of anatomy and cell biology
Street address Yoshida-Konoe-cho, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8501
Country Japan
 
Platform ID GPL1261
Series (2)
GSE4308 Expression data for validation of single cell cDNA amplification method (V1V3 method)
GSE4309 An improved single-cell cDNA amplification method for efficient high-density oligonucleotide microarray analysis
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Model-based Expression Index calculated with dCHIP
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)

Data table
ID_REF VALUE ABS_CALL
AFFX-TrpnX-M_at 24.65366111 A
AFFX-TrpnX-5_at 25.0835563 A
AFFX-TrpnX-3_at 46.96747075 A
AFFX-TransRecMur/X57349_M_at 286.4064157 P
AFFX-TransRecMur/X57349_5_at 370.3562825 P
AFFX-TransRecMur/X57349_3_at 748.7394709 P
AFFX-ThrX-M_at 100.9942957 P
AFFX-ThrX-5_at 104.8345173 P
AFFX-ThrX-3_at 209.728629 P
AFFX-r2-P1-cre-5_at 5539.346231 P
AFFX-r2-P1-cre-3_at 7713.126196 P
AFFX-r2-Ec-bioD-5_at 2011.700753 P
AFFX-r2-Ec-bioD-3_at 1992.273501 P
AFFX-r2-Ec-bioC-5_at 406.3136438 P
AFFX-r2-Ec-bioC-3_at 628.6529579 P
AFFX-r2-Ec-bioB-M_at 205.1478181 P
AFFX-r2-Ec-bioB-5_at 227.5074534 P
AFFX-r2-Ec-bioB-3_at 202.7685369 P
AFFX-r2-Bs-thr-M_s_at 170.700089 P
AFFX-r2-Bs-thr-5_s_at 145.0905754 P

Total number of rows: 45101

Table truncated, full table size 1104 Kbytes.




Supplementary file Size Download File type/resource
GSM98583.CEL.gz 5.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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