|
| Status |
Public on Dec 22, 2015 |
| Title |
Amacr-/- mouse intestine, segment 3, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
mouse intestine, segment 3, Amacr-/-
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype: Amacr-/-
tissue: intestine, segment 3
cell type: endothelial
gender: male
age: 3-3.5 months
|
| Treatment protocol |
The intestine was rinsed with 0.85% NaCl and cut into four equal size segments, and the segments were opened with scissors.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The intestine endothelial layer was collected by scraping and homogenized into TRIzol reagent (Invitrogen). Total RNA was isolated with the MagMAX-96 for Microarrays Total RNA isolation kit (Ambion).
|
| Label |
biotin
|
| Label protocol |
Labelling of the samples was carried out at the Microarray Facility in Leuven (MAF, Leuven, Belgium).
|
| |
|
| Hybridization protocol |
Hydridizations and washing of the chips were carried out at the Microarray Facility in Leuven (MAF, Leuven, Belgium).
|
| Scan protocol |
Scanning of the chips was carried out at the Microarray Facility in Leuven (MAF, Leuven, Belgium).
|
| Description |
knock-out 2, segment 3
|
| Data processing |
The data were collected using the Affymetrix standard protocol and processed and normalized using Chipster Software Suite version 2.0 using Affymetrix default analysis settings and RMA as normalization method. Variances were not stabilized.
|
| |
|
| Submission date |
Aug 14, 2012 |
| Last update date |
Dec 22, 2015 |
| Contact name |
Kaija J Autio |
| E-mail(s) |
kaija.autio@oulu.fi
|
| Phone |
+358 294 48 1142
|
| Organization name |
University of Oulu
|
| Department |
Faculty of Biochemistry and Molecular Medicine
|
| Street address |
P.O.Box 5400
|
| City |
University of Oulu |
| ZIP/Postal code |
90014 |
| Country |
Finland |
| |
|
| Platform ID |
GPL14661 |
| Series (2) |
| GSE40124 |
Expression data from Amacr knock-out mouse intestine |
| GSE40125 |
Expression data from Amacr knock-out mouse liver and intestine |
|
