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Sample GSM98755 Query DataSets for GSM98755
Status Public on Feb 21, 2007
Title DHA-treated cardiomyocyte (DHA-1)
Sample type RNA
 
Channel 1
Source name rat cardiomyocytes
Organism Rattus norvegicus
Characteristics Cy3
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name rat cardiomyocytes treated with DHA
Organism Rattus norvegicus
Characteristics Cy5
Extracted molecule total RNA
Label Cy5
 
 
Description Primary cardiomyocyte cultures were obtained from the ventricles of newborn Wistar rats by the method of Yagev et al.: some cells were grown in HAM F10 plus 10% fetal calf serum and 10% horse serum medium (controls), others in the same medium supplemented with 60 M Eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA). Media were changed every 48 hrs; cells were grown until complete confluence, then they were scraped off in ice cold PBS, and RNA isolation, labeling of complementary RNA (cRNA), hybridization to 22K-gene arrays (Rat oligo array G4130A) and assessment of expression ratios were performed. About one million cells treated with RNAlater were homogenized and total RNA was extracted by column technology (Rneasy Protect mini kit) and analyzed on both a spectrophotometer and Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA). Only samples with 28S/18S ratio of >2.0 and no evidence of ribosomal peak degradation were included. The cRNA was generated by in vitro transcription with the use of T7 RNA polymerase (Low RNA input fluorescent linear amplification kit) and labeled with Cy3-CTP or Cy5-CTP.
Data processing Agilent FeatureExtraction software, Bioconductor packages
 
Submission date Mar 03, 2006
Last update date Feb 21, 2007
Contact name Annalisa Astolfi
E-mail(s) annalisa.astolfi@unibo.it
Phone +39 051 2144633
Organization name University of Bologna
Department "Giorgio Prodi" Cancer Research center
Lab Pediatric Oncology and Hematology lab
Street address via massarenti 11
City Bologna
ZIP/Postal code 40138
Country Italy
 
Platform ID GPL890
Series (1)
GSE4327 Modulation of cardiomyocyte gene expression by n-3 PUFA supplementation

Data table header descriptions
ID_REF
VALUE Log2 of ratio ch2/ch1
CH1_SIG_MEAN gMeanSignal
CH2_SIG_MEAN rMeanSignal
CH1_BKG_MEAN gBGMeanSignal
CH2_BKG_MEAN rBGMeanSignal

Data table
ID_REF VALUE CH1_SIG_MEAN CH2_SIG_MEAN CH1_BKG_MEAN CH2_BKG_MEAN
1 2268.284 60.40175 42.16109 46.36018
2 68.20354 50.03982 39.93004 46.60359
3 -0.088982957 110.4828 57.93534 40.18959 46.53439
4 -0.021455948 123.2328 65.84914 40.34263 46.6205
5 -0.100363639 123.5639 62.81498 40.05026 46.72663
6 -0.217974686 155.2743 71.01266 39.40446 46.92143
7 2147.128 60.96347 39.5966 46.67979
8 -0.364780355 194.2596 86.44255 38.33128 46.4
9 0.040192279 101.6287 58.80591 38.90018 46.31105
10 -2.073539246 864.9918 198.0288 38.47482 46.2446
11 -0.122091984 119.594 60.38889 38.89398 46.62534
12 -0.276093954 163.6549 72.18142 38.81818 46.51159
13 -1.162299354 338.4545 110.5636 39.08795 46.3606
14 2119.943 59.10088 39.09302 46.45617
15 -2.540705703 11152.83 2348.822 39.18262 46.2367
16 -0.229132485 145.5975 66.27542 39.24434 45.61448
17 -0.092464921 134.5339 67.61441 39.18042 45.87035
18 0.102503754 91.87013 56.54545 39.83199 45.66217
19 -0.324087181 193.9956 89.71616 40.271 46.17344
20 0.092229303 86.75225 53.81532 41.63922 45.85014

Total number of rows: 22575

Table truncated, full table size 1123 Kbytes.




Supplementary data files not provided

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