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Sample GSM988371 Query DataSets for GSM988371
Status Public on Jul 17, 2013
Title muscle knock out replicate3
Sample type RNA
 
Source name muscle, knock out
Organism Mus musculus
Characteristics tissue: muscle
genotype: ClpP KO
genetic background: C57BL/6
Growth protocol Mice were housed in Type II L cages (365x207x140 mm, floor area 530 cm2; IVC-based)at an environmental temperature 20-24°C, humidity: 55±10%and under 12/12 h light/dark cycle with food and water ad libitum.
Extracted molecule total RNA
Extraction protocol RNA was extracted by using RNeasy Midi Kit (Qiagen) following manufacturers instructions for animal tissues.
Label biotin
Label protocol Biotinylated cRNA were prepared using the 3'IVT Express Kit (Ambion) from 100ng total RNA according to the manufacturer.
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HT MG-430 PM Array Plates. Plates were hybridized, washed and stained in the Affymetrix Gene Titan.
Scan protocol GeneChips were scanned using the Affymetrix Gene Titan instrument with AGCC 3.0 software.
Data processing The data were analysed using Bioconductor package 'oligo' under R-2.14.0. Background correction, normalization and probe summarization was performed with RMA.
 
Submission date Aug 17, 2012
Last update date Jul 17, 2013
Contact name Michael H. Walter
E-mail(s) michael.walter@agilent.com
Organization name University of Tuebingen
Department Department of Medical Genetics
Lab The Microarray Facility Tübingen
Street address Calwer Str. 7
City Tuebingen
ZIP/Postal code 72076
Country Germany
 
Platform ID GPL11180
Series (1)
GSE40207 Expression data from mitochondrial protease ClpP knock-out mice.

Data table header descriptions
ID_REF
VALUE RMA log2 transformed signal values.

Data table
ID_REF VALUE
1415670_PM_at 7.353661381
1415671_PM_at 7.952897527
1415672_PM_at 8.369933078
1415673_PM_at 7.195965735
1415674_PM_a_at 7.495157144
1415675_PM_at 5.616645815
1415676_PM_a_at 9.714337094
1415677_PM_at 7.376625435
1415678_PM_at 10.38108722
1415679_PM_at 9.932445501
1415680_PM_at 6.977617104
1415681_PM_at 9.014038168
1415682_PM_at 6.523886709
1415683_PM_at 8.832914432
1415684_PM_at 6.472326029
1415685_PM_at 7.611052991
1415686_PM_at 7.951027736
1415687_PM_a_at 9.862458405
1415688_PM_at 10.29450634
1415689_PM_s_at 7.372887891

Total number of rows: 45141

Table truncated, full table size 1159 Kbytes.




Supplementary file Size Download File type/resource
GSM988371_E12R016c03.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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