|
| Status |
Public on Jul 17, 2013 |
| Title |
brain wild type replicate2 |
| Sample type |
RNA |
| |
|
| Source name |
brain, wild type
|
| Organism |
Mus musculus |
| Characteristics |
tissue: brain
genotype: WT
genetic background: C57BL/6
|
| Growth protocol |
Mice were housed in Type II L cages (365x207x140 mm, floor area 530 cm2; IVC-based)at an environmental temperature 20-24°C, humidity: 55±10%and under 12/12 h light/dark cycle with food and water ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted by using RNeasy Midi Kit (Qiagen) following manufacturers instructions for animal tissues.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using the 3'IVT Express Kit (Ambion) from 100ng total RNA according to the manufacturer.
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix HT MG-430 PM Array Plates. Plates were hybridized, washed and stained in the Affymetrix Gene Titan.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Gene Titan instrument with AGCC 3.0 software.
|
| Data processing |
The data were analysed using Bioconductor package 'oligo' under R-2.14.0. Background correction, normalization and probe summarization was performed with RMA.
|
| |
|
| Submission date |
Aug 17, 2012 |
| Last update date |
Jul 17, 2013 |
| Contact name |
Michael H. Walter |
| E-mail(s) |
michael.walter@agilent.com
|
| Organization name |
University of Tuebingen
|
| Department |
Department of Medical Genetics
|
| Lab |
The Microarray Facility Tübingen
|
| Street address |
Calwer Str. 7
|
| City |
Tuebingen |
| ZIP/Postal code |
72076 |
| Country |
Germany |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE40207 |
Expression data from mitochondrial protease ClpP knock-out mice. |
|
