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Sample GSM989179 Query DataSets for GSM989179
Status Public on Sep 20, 2013
Title Subject LST022, biological rep2
Sample type RNA
 
Source name Post
Organism Homo sapiens
Characteristics response: DR
site: Laval
allergen: Cat Pelt
Sex: F
ethnicity: Caucasian
age: 27
mch pc20 (mg/ml) at pre-challenge: 0.63
mch pc20 (mg/ml) at post-challenge: 0.12
% fall in fev1 (early): -48.3
% fall in fev1 (late): -25.8
erythrocytes (x10^12/l): 4.22
hemoglobin (g/l): 120
hematocrit (l/l): 0.358
mean cell volume (fl): 84.8
mean cell hb (pg): 28.4
mean cell hb conc. (g/l): 335
red cell distr. width (%): 13.2
platelets (x10^9/l): 262
leukocytes (x10^9/l): 5.2
neutrophils (x10^9/l): 2.6
lymphocytes (x10^9/l): 1.9
monocytes (x10^9/l): 0.4
eosinophils (x10^9/l): 0.2
basophils (x10^9/l): 0
relative.neutrophils: 0.504
relative.lymphocytes: 0.359
relative.monocytes: 0.086
relative.eosinophils: 0.045
relative.basophils: 0.006
tissue: whole blood
Extracted molecule total RNA
Extraction protocol Samples were stored at -80⁰C until RNA extraction
From PAXgene tube samples, total RNA was purified from 2.5 mL of whole blood using Rneasy mini kit (Qiagen, Chatsworth, CA, USA)
From EDTA tube samples, total RNA was purified from 3.0 mL of whole blood following modified TRIzol-based extraction method.
The yield and quality of RNA was assessed by NanoDrop 8000 Spectrophotometer (Thermo Scientific, Wilmington, DE, USA) and Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA)
Label biotin
Label protocol standard Affymetrix protocol
 
Hybridization protocol Affymetrix Human Gene 1.0 ST array (Affymetrix, Santa Clara, CA)
Scan protocol standard Affymetrix protocol
Data processing Raw data normalization using the 'farms' package consisted of RMA background correction, quantile normalization and summarization of probe-level data using Factor Analysis for Robust Microarray Summarization
Data filtering was also performed using the Informative vs. Non-Informative Calls function in the 'farms' package. Differential gene expression was determined using moderated t-tests in limma using an FDR cut-off of 10%.
All statistical analyses were performed in the statistical computing program R.
 
Submission date Aug 20, 2012
Last update date Sep 20, 2013
Contact name Scott Tebbutt
E-mail(s) scott.tebbutt@hli.ubc.ca
Organization name University of British Columbia
Department Medicine
Lab James Hogg Research Centre
Street address Room 166, 1081 Burrard Street
City Vancouver
State/province BC
ZIP/Postal code V6Z 1Y6
Country Canada
 
Platform ID GPL6244
Series (1)
GSE40240 Expression data from peripheral blood - blood draws at Pre and Post time points of Allergen inhalation challenge (ER and DR)

Data table header descriptions
ID_REF
VALUE RMA normalized

Data table
ID_REF VALUE
7892501 1.820185736
7892502 4.420509617
7892503 5.421789327
7892504 8.676945326
7892505 2.412938677
7892506 4.312933621
7892507 5.302265124
7892508 5.228119612
7892509 8.589685075
7892510 4.831243782
7892511 3.558137797
7892512 7.282880018
7892513 3.592140965
7892514 6.371648618
7892515 9.20199812
7892516 4.911905355
7892517 6.279300836
7892518 2.405613276
7892519 4.87559935
7892520 9.053579797

Total number of rows: 32321

Table truncated, full table size 627 Kbytes.




Supplementary file Size Download File type/resource
GSM989179_LST.022.Pst-DR-PAXgeneRNA_HuGene-1_0-st-v1_.CEL.gz 3.8 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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