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Sample GSM989242 Query DataSets for GSM989242
Status Public on Aug 22, 2012
Title hepatocytes_heat shock_43_biological rep2
Sample type RNA
 
Source name isolated mouse hepatocytes, heat shock: 43°C
Organism Mus musculus
Characteristics strain: FVB/N
gender: male
age: 10-16-week-old
tissue type: liver
cell type: hepatocytes
Treatment protocol heat shock performed in vivo at 43°C for 30 minutes and 2 hours of recovery
Extracted molecule total RNA
Extraction protocol Hepatocytes were isolated (after heat shock and recovery) by collagenase type IA perfusion of the liver
Total RNA was isolated using RNeasy® Mini Kit spin columns (Qiagen) according to the manufacturer's instructions.
Label biotin
Label protocol cDNA was synthesized from 8 ug of total RNA using the One-Cycle cDNA Synthesis Kit with SuperScriptII polymerase then reverse-transcribed with a T7 RNA polymerase incorporating biotin-labeled nucleotides using the GeneChip IVT Labeling Kit.
 
Hybridization protocol Biotin-labeled cRNA probe samples were purified, fragmented and hybridized (without DMSO) at 45°C overnight to the Affymetrix GeneChip® Mouse Genome 430 2.0 Array. The arrays were washed and stained in the Affymetrix Fluidics Station.
Scan protocol The arrays were scanned using a microarray scanner, which is component of the Affymetrix GeneChip Instrument System
Description Gene expression data from isolated somatic cells (hepatocytes) subjected to heat shock at temperature six centigrade's above physiological
Data processing The data was analyzed with R/Bioconductor and Matlab softwares, RMA algorithm with Dai annotation (Brainarray, version 15.1 dated Jan 2012) were used for data normalization.
 
Submission date Aug 21, 2012
Last update date Oct 22, 2012
Contact name Joanna Polanska
E-mail(s) joanna.polanska@polsl.pl
Phone +48322372144
Organization name Silesian University of Technology
Department Automatic Control
Lab Data Mining Group
Street address Akademicka 16
City Gliwice
ZIP/Postal code 44-100
Country Poland
 
Platform ID GPL15967
Series (1)
GSE40248 Expression data from control and heat shocked mouse spermatocytes and hepatocytes

Data table header descriptions
ID_REF
VALUE log2 RMA signal.

Data table
ID_REF VALUE
11287_at 13.29
11298_at 6.57
11302_at 5.72
11303_at 8.83
11304_at 5.65
11305_at 6.99
11306_at 7.25
11307_at 5.79
11308_at 7.14
11350_at 6.55
11352_at 5.98
11354_at 5.11
11363_at 10.76
11364_at 10.54
11370_at 11.39
11409_at 9.54
11416_at 10.35
11418_at 6.18
11419_at 7.01
11421_at 7.41

Total number of rows: 17306

Table truncated, full table size 244 Kbytes.




Supplementary file Size Download File type/resource
GSM989242_HEP_HS_43_2.CEL.gz 3.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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