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Sample GSM991357 Query DataSets for GSM991357
Status Public on Jan 15, 2014
Title 12 months Mouse hipocampus 005
Sample type RNA
 
Source name 12 months Mouse hipocampus
Organism Mus musculus
Characteristics strain b6(%): 62.5
strain sjl(%): 37.5
strain dba2(%): 0
log10(guhcl fractionation a-beta1-40): 4.98311767
transgenic(tg): APP Tg
age: 12 months
tissue: hippocampus
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from mouse and human powdered hippocampi using RNeasy Lipid Tissue Mini kit (Qiagen). cDNA was prepared using High Capacity RNA-to-cDNA Master Mix (#4390775 Applied Biosystems) following the manufacturer’s standard protocol. Quality of RNA was assessed for each sample using an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Illumina® TotalPrep™ RNA Amplification Kit
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description 005
Data processing The data were normalised using rank invariant normalisation with IlluminaGUI in R
 
Submission date Aug 23, 2012
Last update date Jan 15, 2014
Contact name Noriyuki Hayashi
Organization name Osaka University Graduate School of Medicine
Department Complementary and Alternative Medicine
Street address G9, 2-2, Yamadaoka,
City Suita, Osaka
ZIP/Postal code 565-0871
Country Japan
 
Platform ID GPL6885
Series (1)
GSE40330 Abeta levels in APP Tg mice with mixed genetic backgrounds

Data table header descriptions
ID_REF
VALUE rank invariant normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 3615.6 1
ILMN_2721178 1188.5 1
ILMN_3033922 1433.1 1
ILMN_3092673 3961 1
ILMN_2816356 23.2 0.92857
ILMN_2808939 1014.1 1
ILMN_2634564 505.5 1
ILMN_2737647 36 0.98747
ILMN_2734484 48.6 1
ILMN_2952292 62.5 1
ILMN_2699078 3.1 0.22055
ILMN_1213681 281.1 1
ILMN_2735413 15.6 0.77444
ILMN_2735415 16.8 0.80576
ILMN_2891688 250.8 1
ILMN_2637698 671.5 1
ILMN_2674228 340.3 1
ILMN_2601546 11.9 0.66917
ILMN_1230831 18.6 0.8584
ILMN_2848071 43.7 0.99875

Total number of rows: 25697

Table truncated, full table size 586 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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