|
| Status |
Public on Jan 15, 2014 |
| Title |
12 months Mouse hipocampus 011 |
| Sample type |
RNA |
| |
|
| Source name |
12 months Mouse hipocampus
|
| Organism |
Mus musculus |
| Characteristics |
strain b6(%): 16
strain sjl(%): 84
strain dba2(%): 0
log10(guhcl fractionation a-beta1-40): 4.31527236
transgenic(tg): APP Tg
age: 12 months
tissue: hippocampus
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from mouse and human powdered hippocampi using RNeasy Lipid Tissue Mini kit (Qiagen). cDNA was prepared using High Capacity RNA-to-cDNA Master Mix (#4390775 Applied Biosystems) following the manufacturer’s standard protocol. Quality of RNA was assessed for each sample using an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina® TotalPrep™ RNA Amplification Kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
011
|
| Data processing |
The data were normalised using rank invariant normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Aug 23, 2012 |
| Last update date |
Jan 15, 2014 |
| Contact name |
Noriyuki Hayashi |
| Organization name |
Osaka University Graduate School of Medicine
|
| Department |
Complementary and Alternative Medicine
|
| Street address |
G9, 2-2, Yamadaoka,
|
| City |
Suita, Osaka |
| ZIP/Postal code |
565-0871 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE40330 |
Abeta levels in APP Tg mice with mixed genetic backgrounds |
|
