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Sample GSM9916 Query DataSets for GSM9916
Status Public on Feb 06, 2004
Title Control 1st repeat
Sample type RNA
 
Source name Whole seedlings
Organism Arabidopsis thaliana
Extracted molecule total RNA
 
Description Arabidopsis seedlings (1 week-old ) were plated on 0.5 x MS salts and vitamins containing 6 g/L Phytagar (Gibco). Seeds were surface sterilized by a 3 minutes incubation with 50 % ethanol, 0.5 % Triton X-100 followed by a brief rinse with 95 % ethanol. Seeds were air-dried after surface sterilization. Four Falcon grided deep Petri dishes of each genotype, aprox. 200 seeds per plate were used. Petri dishes were cold treated (4 oC) for 48 h and then placed in a growth room [16 h day, 24 oC, 150 mE.m-2.s-1 (E, Einstein; 1 E = 1 mol of photons)] on a stand in a vertical orientation and left undisturbed. After a week, total RNA was extracted with Trizol (Gibco) and further purified with RNeasy columns (Qiagen) following manufacturer instructions. Total RNA was extracted from each genotype and, prior to target preparation, was mixed in equal proportions to make the control 1 sample [(Control 1= wild type + wild type transformed with the empty pART27 vector). Synthesis of double-stranded cDNA, biotin-labeled cRNA, cRNA fragmentation, hybridization, washing, staining and scanning were performed according to GeneChip Expression Analysis Technical Manual (http://www.affymetrix.com), and were performed at the Sequencing Laboratory (Washington State University).
Keywords = AtRALF1-1, RALF, polypeptide
 
Submission date Sep 09, 2003
Last update date May 28, 2005
Contact name Daniel Scherer de Moura
E-mail(s) daniel_moura54@hotmail.com
Phone 509-3351890
Fax 509-3353412
Organization name Institute of Biological Chemistry
Lab Clarence "Bud" Ryan
Street address
City Pullman
State/province WA
ZIP/Postal code 99164-6340
Country USA
 
Platform ID GPL198
Series (1)
GSE641 Control vs. 35S:AtRALF1-1

Data table header descriptions
ID_REF
VALUE Average signal, relative abundance of a transcript, value generated by the Micro Array Analysis Suite 5.1
ABS_CALL P, present; A, absent; M, marginal
QUALITY

Data table
ID_REF VALUE ABS_CALL QUALITY
244901_at 31.9 P 0.01416
244902_at 25.8 A 0.095215
244903_at 40.3 A 0.095215
244904_at 13.8 A 0.366211
244905_at 20.2 A 0.303711
244906_at 98.8 A 0.19458
244907_at 1.6 A 0.828613
244908_at 3.3 A 0.919434
244909_at 26.4 A 0.533936
244910_s_at 17.5 P 0.008057
244911_at 5.9 A 0.633789
244912_at 78.7 A 0.095215
244913_at 7.9 A 0.80542
244914_at 1.9 A 0.870361
244915_s_at 24.3 A 0.432373
244916_at 29.5 A 0.219482
244917_at 1.8 A 0.976074
244918_at 15.2 A 0.466064
244919_at 3.1 A 0.601074
244920_s_at 45 P 0.010742

Total number of rows: 22810

Table truncated, full table size 577 Kbytes.




Supplementary data files not provided

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