|
| Status |
Public on Jun 01, 2013 |
| Title |
WTPA2 |
| Sample type |
RNA |
| |
|
| Source name |
keratinocyte harvest from p1 pups
|
| Organism |
Mus musculus |
| Characteristics |
strain: FVBN
genotype/variation: WT
cell type: keratinocyte
|
| Treatment protocol |
TPA treatment (0.2 μM) for 12 h
|
| Growth protocol |
Keratinocyte media, 37°C, 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
The data were normalised using vsn normalisation with limma package in R
|
| |
|
| Submission date |
Aug 27, 2012 |
| Last update date |
Jun 01, 2013 |
| Contact name |
Annan Yang |
| Organization name |
Johns Hopkins University
|
| Department |
Physiology
|
| Street address |
725 N. Wolfe St, Biophysics 203
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE40382 |
Different response of WT and Ts keratinocyte to TPA treatment |
|
